中国中医药信息杂志2018，Vol.25Issue(1)：69-73,5.DOI:10.3969/j.issn.1005-5304.2018.01.015
基于指纹图谱与一测多评技术分析化学转化法富集甘草药渣总黄酮的研究
Study on Enrichment of Total Flavonoids from Licorice Residue by Chemical Conversion Method Based on Fingerprint and Quantitative Analysis of Multi-components with a Single-marker Technique
摘要
Abstract
Objective To establish a combined quality evaluation model of fingerprint and quantitative analysis of multi-components with a single-marker (QAMS) to analyze the total flavonoids from licorice residue by the chemical conversion method; To provide technical support for quality control in production. Methods Total flavonoids of breaking cell wall and enriching were taken as the object of study to establish fingerprint. With liquiritin as internal reference, the relative correction factors of isoliquiritin, glycyrrhizin, isoliquiritigenin and glycyrrhizic acid were established respectively, and the contents were determined. Meanwhile, the calculated values were compared with the measured value by external standard method to verify the practicability and stability of QAMS. Results The HPLC fingerprint of total flavonoids from licorice residue was established. 11 common peaks were identified, and 5 common peaks were identified, and the similarity of the 10 extracts was >0.99; the relative error between the calculated results of QAMS and the measured values of the external standard method was <4%; the RSD of relative correction factor calculated by the multiple concentration method was <2%. Conclusion The method is accurate, reliable, specific, and stable, with good repeatability, which can be used for the quality control of total flavonoids from licorice residue.关键词
指纹图谱/一测多评/甘草药渣提取物/质量评价Key words
fingerprint/quantitative analysis of multi-components with a single-marker/licorice residue extract/quality evaluation分类
医药卫生引用本文复制引用
刘效栓,肖正国,罗燕燕,李喜香,李季文,毕映燕,刘军刚..基于指纹图谱与一测多评技术分析化学转化法富集甘草药渣总黄酮的研究[J].中国中医药信息杂志,2018,25(1):69-73,5.基金项目
兰州市科技发展计划项目(兰州市科技局2014-2-32) （兰州市科技局2014-2-32）
