中国卒中杂志2017，Vol.12Issue(12)：1097-1103,7.DOI:10.3969/j.issn.1673-5765.2017.12.006
曲克芦丁脑蛋白水解物对大脑中动脉栓塞后大鼠神经血管单元的保护作用
Protective Effect of Troxerutin and Cerebroprotein Hydrolysate on Neurovascular Unit in Rats after Middle Cerebral Artery Occlusion
曾静 1王茹 2李丹丹 2赵弘轶 1张微微 1黄勇华1
作者信息
- 1. 100700 北京 中国人民解放军陆军总医院神经内科
- 2. 陕西中医药大学第二临床医学院
- 折叠
摘要
Abstract
Objective To investigate the protective effect of Troxerutin and Cerebroprotein Hydrolysate on neurovascular units after focal cerebral ischemia, and to explore the mechanism of its protective effects. Methods The middle cerebral artery occlusion (MCAO) was used to induce the focal cerebral ischemia model in rats. A total of 120 male SD rats were randomly divided into sham operation group (n=40), MCAO group (n=40), and MCAO + Troxerutin group (n=40). The MCAO +Troxerutin group was given Troxerutin 3 ml/kg, 1 time per day for 3 days after the operation by intraperitoneal injection. Modified Neurological Severity Score (mNSS) was used to evaluate the behavior changes of 3 d, 7 d and 14 d after embolization. Three days after embolization, the infarct area and blood flow was tested by T2and ASL sequence of 7.0T high resolution MRI. Nissl staining was used to evaluate the survival rate and morphological changes of neurons. Immunofluorescence staining was used to assess the expression of endothelial cells, astrocytes and tight junction markers in each group; furthermore, Western blot was used to evaluate the expression of 3-nitrotyrosine (3-NT), matrix metalloproteinase 9 (MMP-9) and inducible nitric oxide synthase (iNOS) in each group. Results Three days after MCAO, compared with the sham group, the ASL sequence of MCAO group showed a significant reduction of blood flow in the infarct area; nissl staining showed a reduction in neuronal survival, a large number of vacuoles, and pyknosis; immunofluorescence showed that endothelial cells and tight junction markers decreased and astrocytes increased; and Western blot demonstrated that iNOS, 3-NT and MMP-9 increased. While for MCAO + Troxerutin group, the blood flow of infarct area was significant decreased; the survival of neurons, the molecular marker of endothelial cells and tight junction were increased. Astrocytes and iNOS, 3-NT, MMP-9 were significant decreased compared with MCAO group. Conclusion Troxerutin and Cerebroprotein Hydrolysate could inhibit the expression of iNOS and MMP-9 and reduce the production of 3-NT, so as to play a role of protecting the neurovascular cells of MCAO rats.关键词
大脑中动脉栓塞/神经血管单元/血脑屏障/曲克芦丁Key words
Middle cerebral artery occlusion/Neurovascular unit/Blood-brain barrier/Troxerutin引用本文复制引用
曾静,王茹,李丹丹,赵弘轶,张微微,黄勇华..曲克芦丁脑蛋白水解物对大脑中动脉栓塞后大鼠神经血管单元的保护作用[J].中国卒中杂志,2017,12(12):1097-1103,7.
