安徽大学学报（自然科学版）2018，Vol.42Issue(1)：100-108,9.DOI:10.3969/j.issn.1000-2162.2018.01.013
耐盐碱刺槐离体再生快繁体系的建立与优化
Establishment and optimization of rapid regeneration system in vitro for salt-tolerance Robinia pseudoacacia
摘要
Abstract
To improve the efficiency of rapid propagation of salt-tolerance Robinia pseudoacacia and meet requirements of green in coastal and coastal saline area,the effects of the different conditions on plantlet differentiation and rooting were studied using salt-tolerance black locust- "W009" and "W038" as the experimental materials.The results showed that the germination rate of adventitious buds for shoot tip was better than that of stem segments,and the optimum medium of "W009" for adventitious bud germination from shoot tip was MS + 6-BA 0.5 mg · L - 1 + NAA 0.05 mg · L - 1,the medium MS + 6-BA 1.0 mg · L - 1 + NAA 0.1 mg · L - 1 was the best for stem segments ; while the optimal medium for germination of adventitious buds from shoot tips and stem segments of "W038"was MS + 6-BA 1.0 mg · L - 1 NAA 0.1 mg · L - 1.The culture medium of "W009" for adventitious bud proliferation was MS + 6-BA 1.0 mg · L- 1 + NAA 0.05 m · L- 1,and the medium MS + 6-BA 2.0 mg · L- 1 + NAA 0.1 mg · L- 1 was optimal for "W038".The rooting medium 1/2 MS medium containing 0.1 mg · L - 1 IBA,0.2 mg · L - 1 NAA and 1.0 g · L- 1 AC was the most suitable for "W009",and the optimum rooting medium of "W038"was 1/2 MS medium combined with 0.3 mg · L- 1 IBA,0.2 mg · L- 1 NAA and 1.0 g · L- 1 AC.关键词
耐盐碱刺槐/快繁再生/分化/生根Key words
salt-tolerance Robinia pseudoacacia/rapid regeneration/differentiation/rooting分类
农业科技引用本文复制引用
王兴翠,林桂玉,乔宁,曹帮华..耐盐碱刺槐离体再生快繁体系的建立与优化[J].安徽大学学报（自然科学版）,2018,42(1):100-108,9.基金项目
潍坊科技学院博士基金启动项目(W13K006) （W13K006）
潍坊市科学技术发展计划项目(201301160) （201301160）
山东省重点研发计划项目(2015GNC111026) （2015GNC111026）
山东省良种工程资助项目(2016LZGC002) （2016LZGC002）
山东省农业科技成果转化资金资助项目 （）
