北京中医药大学学报2017，Vol.40Issue(12)：1004-1010,7.DOI:10.3969/j.issn.1006-2157.2017.12.007
健脾益气摄血方及其拆方对辛伐他汀诱导斑马鱼脑出血的防治作用研究
Preventive and therapeutic effects of Jianpi Yiqi Shexue Keli and its disas-sembled prescriptions in treating zebrafish cerebral hemorrhage induced by simvastatin
摘要
Abstract
Objectives To explore the preventive and therapeutic effects of Jianpi Yiqi Shexue Keli ( Spleen-fortifying Qi-tonifyingHemostatic Granule, JPYQSXKL) and its disassembled prescriptions ( Jianpi Granules) in the treatment of zebrafish cerebral hemorrhage induced by simvastatin. Methods 660 mela-nin alleles mutant Albino zebrafishes were randomly divided into normal control group, model group, Jianpi groups ( A, B, C, D) and subgroups of their different concentration (100 μg/mL, 250 μg/mL, 500 μg/mL, 750 μg/mL, and 1000 μg/mL) ( n=30 in each group) . Blinding measures were strictly followed throughout the process. The model of intracranial hemorrhage was established by adding 0. 5μM simvastatin while administering granules of different concentration at the same time. 24 hours later, 20 fi-shes out of each group were observed under the microscope to count the number of hemorrhagic fishes ( S) and thus calculate hemorrhage percentage and its decrease rate. Results ①The incidence rate of hemorrhage in Jianpi groups (100 μg/mL, 250 μg/mL, 500 μg/mL and 750 μg/mL) was 75％, 70％, 60％ and 70％;②The incidence rate of hemorrhage in Jianpi Yiqi groups (100 μg/mL, 250 μg/mL and 500 μg/mL) were 60％, 40％ and 85％; ③The incidence rate of hemorrhage in Jianpi Yiqi Shengxue (Spleen-fortifying Qi-tonifying Blood-nourishing) groups (100 μg/mL and 250 μg/mL)were 50％ and 45％;④The incidence rate of hemorrhage in Jianpi Yiqi Shexue groups(100 μg/mL, 250 μg/mL and 500 μg/mL) were 35％, 25％ and 45％. Conclusions No protective effects of Jianpi granule and Jianpi Yiqi granule on intracranial hemorrhage induced by simvastatin in zebrafish were observed. Jianpi Yiqi Shengxue granule and Jianpi Yiqi Shexue granule appeared to have a good effect yet high dose was toxic.关键词
健脾益气摄血方/脑出血/出血减少率/斑马鱼Key words
Jianpi Yiqi Shexue Granule/cerebral hemorrhage/hemorrhage decrease rate/zebrafish分类
医药卫生引用本文复制引用
李潇,朱长乐,赵宁,杨璐,陈信义,刘莉..健脾益气摄血方及其拆方对辛伐他汀诱导斑马鱼脑出血的防治作用研究[J].北京中医药大学学报,2017,40(12):1004-1010,7.基金项目
国家重点基础研究发展计划(973计划)资助项目(No. 2013CB531705) National Basic Research Program of China(No. 2013CB531705) （973计划）
