北京中医药大学学报2017，Vol.40Issue(12)：1011-1017,7.DOI:10.3969/j.issn.1006-2157.2017.12.008
培元解郁方对糖皮质激素诱导大鼠抑郁模型TRP-KYN代谢的影响及神经元可塑性调节机制
Effects of Peiyuan Jieyu Formula on TRP-KYN metabolic pathway and its neuron protection mechanism in depressed rats induced by glucocorti-coid
摘要
Abstract
Objective To study the effects of Peiyuan Jieyu Formula ( PYJYF) on rats treated with glu-cocorticoid, its influences on metabolism of the TRP-KYN pathway and the impacts on hippocampal and cortical neuronal plasticity. Methods 70 rats were randomly divided into the following 7 groups ( n=10 in each group): normal blank group, model group, fluoxetine group, mid- and high-dose Tiansi Yin ( TSY) groups, Sini San ( SNS) groups, and PYJYF groups. Rat model of depression was established by intraperitoneal injection of glucocorticoid for 28 days. Sucrose solution consumption and Open-field test were conducted. The content of TRP, KYN, KA and Q in serum was measuredby using HPLC-MS/MS technique. The mRNA expressions of NR and BDNF in brain tissue were measured by using RT-PCR. Results 28 days after the injection of glucocorticoid in model group, the consumption of sucrose solution and total score in open-field test was significantly decreased ( P <0 . 05 ) . The ratios of KYN/TRP and KYN/KA increased significantly ( P<0 . 05 ) . The mRNA expression level of BDNF in hippocampal de-creased significantly ( P<0 . 05 ) . The mRNA expression level of NR increased significantly ( P<0 . 05 ) . Peiyuan Jieyu Formula increased the consumption of sucrose solution (P<0. 05),the total score in open-field test,the ratios of KYN/Q and the mRNA expression level of BDNF in hippocampus(P<0. 05) when compared with the model group. The ratios of KYN/TRP and KYN/KA(P<0. 05) and the mRNA ex-pression level of NR in cortex ( P <0. 05 ) of Peiyuan Jieyu Formula group decreased. Conclusion Peiyuan Jieyu Formula can reduce the metabolism of TRP-KYN, inhibit neurotoxicity, improve neural protection, and promote the hippocampal and cortical neural plasticity.关键词
糖皮质激素/TRP-KYN代谢/海马/皮层/神经可塑性/大鼠Key words
glucocorticoid/TRP-KYN metabolic pathway/hippocampus/cortex/neural plasticity/rats分类
医药卫生引用本文复制引用
李海娜,李丽娜,王淑艳,范盎然,于雪,王萍萍,刘保秀,畅洪昇..培元解郁方对糖皮质激素诱导大鼠抑郁模型TRP-KYN代谢的影响及神经元可塑性调节机制[J].北京中医药大学学报,2017,40(12):1011-1017,7.基金项目
国家自然科学基金资助项目( No. 81373584) Project sponsored by National Natural Science Foundation (No. 81373584) （ No. 81373584）
