森林与环境学报2018，Vol.38Issue(1)：1-6,6.DOI:10.13324/j.cnki.jfcf.2018.01.001
杉木纤维素合酶(ClCesA2)基因的克隆与表达分析
Cloning and expression analysis of cellulose synthase ClCesA2 from Cunninghamia lanceolata
摘要
Abstract
In order to explore the mechanism of wood development of Chinese fir, total RNA extracted from Chinese fir seedlings was used for RT-PCR, then the full-length open reading frame sequence of ClCesA2 gene was amplified.Sequencing and analysis of the gene showed that the full length was 3276 bp, encoding 1091 amino acids, containing zinc finger structure, eight cross membranes and a conservative DX??QVLRW structure domain.The qPCR result revealed that the gene highly expressed in the stem, followed by root and leaf. Phylogenetic tree analysis showed that the gene was clustered with genes involved in the synthesis of the primary wall which inferred that the ClCesA2 gene may play an important role in the process of cellulose synthesis of the primary cell wall.关键词
杉木/纤维素合酶基因/克隆/序列分析Key words
Cunninghamia lanceolata ( Lamb.) Hook./cellulose synthase gene/cloning/sequence analysis分类
资源环境引用本文复制引用
陈潇潇,曹光球,汪凤林,罗红艳,魏晓骁,曹世江..杉木纤维素合酶(ClCesA2)基因的克隆与表达分析[J].森林与环境学报,2018,38(1):1-6,6.基金项目
国家重点研发项目"杉木高效培育技术研究"(KCa16031A) （KCa16031A）
福建农林大学校重点项目建设(6112C039B) （6112C039B）
福建农林大学林学院青年基金项目(k13xjj02a). （k13xjj02a）
