森林与环境学报2018，Vol.38Issue(1)：7-12,6.DOI:10.13324/j.cnki.jfcf.2018.01.002
不同逆境胁迫下杉木Cu/Zn-SOD基因表达分析
Analysis of Cu/Zn-SOD gene expression in Chinese fir under different stress
摘要
Abstract
Adverse stress affects plant growth and development, and moderate stress can promote the synthesis and accumulation of some secondary metabolites, which would greatly improve the quality of plant.Under stress treatment, superoxide dismutase ( SOD) plays a key role in protecting cells from reactive oxygen speices damage by the mediation of reactive oxygen scavenging.In order to reveal the Cu/Zn-SOD gene in Chinese fir stress effect, the expression pattern of Cu/Zn-SOD gene in Chinese fir seedlings were analyzed under low temperature of 4℃, mannitol 0.0546 g· mL-1 , aluminum ion 2 mmol· L-1 , and 300 mmol· L-1 NaCl stress using qRT-PCR technology, and the variation of SOD activity under different treatments were measured.The results showed that the Cu/Zn-SOD gene in tissue culture seedlings of Chinese fir have changed compared with CK under different stress conditions, and total expression showed a trend of increasing first and then decreasing.The gene expression reached the highest in the low temperature of 4℃, mannitol 0.0546 g· mL-1 , aluminum ion 2 mmol· L-1 , and 300 mmol· L-1 NaCl stress were 48, 48, 16 and 24 h which were 6.4, 5.3, 6.4, 10.4 times, respectively, and compared with the control group, Cu/Zn-SOD gene expression reached significant level for the different stress.SOD also showed similar changes trend, but were higher than CK which may related to increase of SOD membergene. In conclusion, Cu/Zn-SOD gene plays an important role in the regulation of Chinese fir seedlings in adverse stress which would provides a theoretical basis for resistance breeding for further understanding of molecular mechanism and stress in Chinese fir Cu/Zn-SOD mechanism.关键词
杉木组培苗/Cu/Zn-SOD/qRT-PCR/SOD活性/基因表达分析Key words
Chinese fir tissue culture seeding/Cu/Zn-SOD/qRT-PCR/SOD activity/gene expression analysis分类
农业科技引用本文复制引用
饶丽莎,许珊珊,黄田盛,王培,曹光球,林思祖..不同逆境胁迫下杉木Cu/Zn-SOD基因表达分析[J].森林与环境学报,2018,38(1):7-12,6.基金项目
国家林业局杉木工程技术研究中心平台建设项目(ptjh130002) （ptjh130002）
中央财政支持地方专项(6213c0111) （6213c0111）
福建农林大学校重点项目建设专项:人工林培育学科建设(6112c0310) （6112c0310）
林学院学科建设基金项目(6112c0300). （6112c0300）
