森林与环境学报2018，Vol.38Issue(1)：20-26,7.DOI:10.13324/j.cnki.jfcf.2018.01.004
杉木愈伤组织的继代增殖与植株再生
Callus subculture and regeneration of Cunninghamiaa lcneoltaa
摘要
Abstract
In order to provide efficient receptor system for genetic engineering of somatic cell hybridization and genetic transforma-tion, callus proliferation and plant regeneration system of Cunninghamia lanec olata ( Lamb.) HooK was built.Using the callus which induction from the cotyledon and hypocotyl as materials, the effects of the following factors including different types of hormone concentrations and combinations on callus subculture and plant regeneration were investigated.The results indicated that different hormone combinations have a significant influence on callus subculture.2,4-D is helpful to reduce the browning phenomenon in subculture process.NAA and KT are beneficial to the callus subculture.The optimal medium for callus subculture was DCR+6-BA 1.5 mg· L-1+KT 1.0 mg· L-1+NAA 1.0 mg· L-1 and DCR+6-BA 2.0 mg· L-1+KT 1.5 mg· L-1+NAA 1.5mg · L-1 .The optimal culture conditions were dark and 800lx intensity for callus subculture .Callus were most suitable for transfer to new medium after subculture of 30-45 d.The optimal medium for callus induction regeneration bud was MS+6-BA 0.5 mg· L-1 +KT 1.5 mg· L-1 , the induction rates was 80％.The number of buds regenerated from the callus after one subculture was more than that of the callus without subculture, the average number was 3-4.The optimal medium for rooting of regenerated plant was 1/2MS+IBA 1.0 m·g L-1 in a rate of 89.5％.关键词
杉木/愈伤组织/继代培养/植株再生Key words
Cunninghamia lacn eolata ( Lamb.) Hook./callus/subculture/plant regeneration分类
农业科技引用本文复制引用
黄娟,吴鹏飞,程浩,马祥庆..杉木愈伤组织的继代增殖与植株再生[J].森林与环境学报,2018,38(1):20-26,7.基金项目
国家重点研发计划项目(2016YFD0600301) （2016YFD0600301）
福建农林大学科技发展基金项目(KF2015036). （KF2015036）
