森林与环境学报2018，Vol.38Issue(1)：33-37,5.DOI:10.13324/j.cnki.jfcf.2018.01.006
油茶种仁蛋白双向电泳体系的建立
Two-dimensional gel electrophoresis system establishment of Camellia oleifera kernel
摘要
Abstract
Using kernel of Camellia oleifera 'Min 43 ' , a proper two-dimensional electrophoresis system for kernel of Camellia oleifera Abel.was optimized by three factors:sample preparation methods, IEF focusing program parameter settings and SDS-PAGE gel concentration.The results showed that more clear spots could be obtained by the method of TCA/acetone combined with SDS phenol extraction, the isoelectric focusing of 62000 V· h was more suitable for the separation of protein and the concentration of 10％SDS gel was better for the separation kernel of C.oleifera protein.The result of this research laid the foundation for the following proteomics research in oil tree.关键词
油茶种仁/蛋白/双向电泳/体系Key words
Camellia oleifera kernel/protein/two-dimensional electrophoresis/system分类
农业科技引用本文复制引用
何艺凡,宋倩倩,林文俊,陈世品,陈辉,郑国华..油茶种仁蛋白双向电泳体系的建立[J].森林与环境学报,2018,38(1):33-37,5.基金项目
高等学校博士学科点专项科研基金项目(20123515110010) （20123515110010）
中央财政林业科技推广示范项目(2017). （2017）
