口腔疾病防治2017，Vol.25Issue(12)：774-778,5.DOI:10.12016/j.issn.2096-1456.2017.12.005
肥胖复合牙周炎大鼠骨骼肌中瘦素受体表达的组织学研究
Histological observation of leptin receptor in skeletal muscle in obese rats with periodontitis
摘要
Abstract
Objective To invesitgate the protein expression for leptin receptor in skeletal muscle in periodontitis and obese rat models,with the aim of exploring the effect of experimental periodontitis on adipokines receptors in insulintargeting tissues and underlying mechanisms of skeletal muscle insulin resistance.Methods Forty natal SD male rats were randomly divided into four groups,respectively:obese rats with periodontitis (OB+ CP group),obese rats without periodontitis (OB group),normal rats with periodontitis (CP group) and normal rats without periodontitis (C group).Obese rat model was established by subcutaneous injection of sodium glutamate.Experimental periodontitis was induced by ligation and inoculation of periodontal pathogens.Immunohistochemical analysis of leptin receptor expression in skeletal muscle of four groups was employed.Results In the skeletal muscle,the protein for leptin receptor in the C group and CP group were popular and strong positive,and there was no statistically significant difference between the groups (P ＞ 0.05).The protein expressions in the OB + CP group and OB group were decreased.Fewer protein expression was detected in the OB + CP group when compared with OB group (P ＜ 0.05).A synergistic interaction between obesity and periodontitis was evident to the expression for the leptin receptor (P ＜ 0.05).Conclusion Local infection induced by experimental periodontitis can barely down-regulate the protein expression for leptin receptor in the skeletal muscle.However,periodontitis can significantly down-regulate the protein expressions with the presence of obesity.关键词
牙周炎/瘦素受体/骨骼肌/胰岛素抵抗Key words
Periodontitis/Leptin receptor/Skeletal muscle/Insulin resistance分类
医药卫生引用本文复制引用
苏媛,吕影涛,张雪洋,陈璇君,章锦才..肥胖复合牙周炎大鼠骨骼肌中瘦素受体表达的组织学研究[J].口腔疾病防治,2017,25(12):774-778,5.基金项目
国家自然科学基金项目(81400509) （81400509）
