广东药科大学学报2017，Vol.33Issue(6)：713-718,6.DOI:10.16809/j.cnki.2096-3653.2017091203
基于析因设计优化复方乙酸曲安奈德洗剂中药物的稳定性
Simultaneous determination of three drug contents in compound triamcinolone acetonide lotion and improvement of drug stability by factorial design
万君晗 1王荣侃 2曾维国 2李添鹏 2龙晓英3
作者信息
- 1. 广东药科大学 药学院,广东 广州 510006
- 2. 中山市第二人民医院 药剂科,广东 中山 528400
- 3. 广东药科大学 中药学院,广东 广州 510006
- 折叠
摘要
Abstract
Objective To establish an HPLC method for simultaneous determination of the content of triamcinolone acetonide acetate, chloramphenicol and salicylic acid in lotion. The factorial design was used to optimize the prescription in order to improve the drug stability. Methods Waters SunFireTM C18 column ( 4.6 mm×150 mm, 5 μm) was used, the mobile phase consisted of 0.1％ phosphoric acid aqueous solution-methanol ( 40 :60) with the flow rate of 1.0 mL/ min, the sample volume of 10 μL, the detection wavelength of 254 nm and the column temperature of 30 ℃ . The degree of drug degradation under high temperature ( 60 ℃ , 10 days) was taken as the index. The factorial design was used to study the effect of the pH, antioxidant and NaCl on the stability of the preparation to optimize the prescription. Results The linear range of triamcinolone acetonide acetate, chloramphenicol and salicylic acid was 2. 5 - 15, 25 - 150 and 60-360 μg/ mL respectively. The average recovery was ( 100.7±1.7) ％, ( 100.6±1.7) ％ and ( 100.2±1.2) ％, respectively. The factor of pH was determined, and the best pH was 4.5. Conclusion The HPLC method established can be used for simultaneous determination of three drugs in lotion, and the compound triamcinolone acetonide acetate lotion with good stability was obtained by controlling the pH of the lotion.关键词
乙酸曲安奈德/氯霉素/水杨酸/稳定性/析因设计Key words
triamcinolone acetonide acetate/chloramphenicol/salicylic acid/stability/factorial design分类
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万君晗,王荣侃,曾维国,李添鹏,龙晓英..基于析因设计优化复方乙酸曲安奈德洗剂中药物的稳定性[J].广东药科大学学报,2017,33(6):713-718,6.
