广东药科大学学报2017，Vol.33Issue(6)：732-736,5.DOI:10.16809/j.cnki.2096-3653.2017101002
赤芍总黄酮提取及纯化工艺研究
Study on the extraction and purification process of total flavonoids from Radix Paeoniae Rubra
杨健峰 1万君晗 2吴永秋 1黄蓓 1黄传利 2张彩凤 2龙晓英1
作者信息
- 1. 广东药科大学 中药学院,广东 广州 510006
- 2. 广东药科大学 药学院,广东 广州 510006
- 折叠
摘要
Abstract
Objective To investigate the extraction and purification process of total flavonoids from Radix Paeoniae Rubra. Methods With the yield of total flavonoids as the evaluation index, single factor and the orthogonal design were used to optimize the extraction process of total flavonoids including extraction duration, extraction temperature, solid-liquid ratio and the extraction times. D-101 resin was used to optimize the purification process of total flavonoids by measuring the adsorption ratio, eluting ratio of total flavonoids and so on. Results The optimum extract parameters were as follows, temperature of 60 ℃ , solid-liquid ratio of 1 : 12, extraction for twice and 1.5 h each time. The content of total flavonoids in Radix Paeoniae Rubra was 1.82％. The optimum purification conditions were as follows, resin-sample ratio of 1 :1, 0.5 mg/ mL sample concentration, 3 BV sample volume, 60％ eluting reagent concentration, 2 BV/ h flow rate, 3 BV eluting reagent, and diameter to height ratio of 1 :9. The dry extract yield in purified substance reduced from 8.99％ to 2.49％ and the total flavonoids content increased from 185.76 mg/ g to 689.67 mg/ g. Conclusion The optimum extraction and purification process was economical, stable and feasible and could provide reference for the research and development of Radix Paeoniae Rubra.关键词
赤芍/总黄酮/正交试验/提取工艺/纯化工艺Key words
Radix Paeoniae Rubra/total flavonoids/orthogonal design/extraction process/purification process分类
医药卫生引用本文复制引用
杨健峰,万君晗,吴永秋,黄蓓,黄传利,张彩凤,龙晓英..赤芍总黄酮提取及纯化工艺研究[J].广东药科大学学报,2017,33(6):732-736,5.
