广东药科大学学报2017，Vol.33Issue(6)：748-752,5.DOI:10.16809/j.cnki.2096-3653.2017091901
HPLC法同时测定宝咳宁颗粒中4种黄酮苷类成分
Simultaneous determination of four flavonoid glycosides in Baokening granules by HPLC
摘要
Abstract
Objective To establish an HPLC method for simultaneous determination of naringin, neohesperidin, baicalin, and wogonoside in Baokening granules. Methods The analysis was performed on a Dikma Spursil C18 column ( 4.6 mm×250 mm, 5 μm) with its temperature maintained at 30 ℃ , mobile phase comprising of acetonitrile-0.1％ H3 PO4 with gradient elution at the flow rate of 1.0 mL/ min. The detection wavelengths were set at 283 nm and 275nm. Results Four constituents showed good linear relationships ( r > 0. 9990) , whose average recoveries were 95. 7％ ( RSD = 1. 93％) , 95. 4％ ( RSD =2.10％) , 103. 5％ ( RSD = 1. 57％) , and 102. 4％ ( RSD = 2. 50％) , respectively. Conclusion The established method was proved to be accurate, reliable, and repeatable, which was suitable for the quality control of Baokening granules.关键词
宝咳宁颗粒/柚皮苷/新橙皮苷/黄芩苷/汉黄芩苷/HPLC法Key words
Baokening granules/naringin/neohesperidin/baicalin/wogonoside/HPLC分类
医药卫生引用本文复制引用
陈淑婷,宋粉云,高晓霞,钟兆健..HPLC法同时测定宝咳宁颗粒中4种黄酮苷类成分[J].广东药科大学学报,2017,33(6):748-752,5.基金项目
上海市药物(中药)代谢产物研究重点实验室开放课题(2015SHDX1001) （中药）
