广东药科大学学报2017，Vol.33Issue(6)：768-772,5.DOI:10.16809/j.cnki.2096-3653.2017101601
原花青素对H2 O2诱导SH-SY5Y细胞氧化损伤的保护作用
Protection of proanthocyanidins on oxidative damage in SH-SY5Y cells induced by H2 O2
摘要
Abstract
Objective To study the protection and mechanism of proanthocyanidins on oxidative damage in SH-SY5Y cells induced by H2 O2 . Methods MTT method was used to select the useful concentration of proanthocyanidins. The model of oxidative damage on SH-SY5Y cells was established with H2 O2 ( 400μmol/ L) treatment. The cells were pretreated with 6. 25, 12. 5 and 25 μg/ mL proanthocyanidins. The changes of SH-SY5Y cell morphology were observed by inverted microscope. MTT method was used to detect the survival rate of cells, and the expression of HSPA1L protein was detected by western blot. Results Compared with the model group, the cell morphology and survival rate were significantly improved after proanthocyanidins treatment in a dosage-dependent manner. Meantime, the level of HSPA1L was significantly decreased in 25 μg/ mL proanthocyanidins group. Conclusion Proanthocyanidins can protect SH-SY5Y cells from oxidative damage induced by H2 O2 , and its mechanism may be related to modulate HSPA1L expression.关键词
原花青素/H2O2/SH-SY5Y细胞/HSPA1L蛋白Key words
proanthocyanidins/hydrogen peroxide/SH-SY5Y cells/HSPA1L分类
医药卫生引用本文复制引用
杨霞,胡苗,何茵如,焦萌亚,梅寒芳..原花青素对H2 O2诱导SH-SY5Y细胞氧化损伤的保护作用[J].广东药科大学学报,2017,33(6):768-772,5.基金项目
国家自然科学基金项目(81401150) （81401150）
