海南医学院学报2017，Vol.23Issue(24)：3331-3334,4.DOI:10.13210/j.cnki.jhmu.20171218.002
毛脉酸模正丁醇部位对内毒素所致小鼠急性肺损伤的影响
Effects of n-butanol extract of Rumex gmelini Turcz on the endotoxin-induced acute lung inju-ry in mice
摘要
Abstract
Objective:To study the effects of n-butanol extract of Rumex gmelini Turcz on the endotoxin-induced acute lung injury(ALI)in mice.Methods:Kunming mice were selected as experimental animals and randomly divided into normal control group(NC group),acute lung injury group(ALI group)and n-butanol extract of Rumex gmelini Turcz group(Rum group).ALI group and Rum group were established into ALI models by intraperitoneal injection of endotoxin,and Rum group were given intragastric administration of n-butanol extract of Rumex gmelini Turcz for intervention before model establishment. 12h after endotoxin injection,the superior lobe of right lung was taken to determine the water content,and the inferior lobe of right lung was taken to determine the contents of AQPs molecules,inflammatory response molecules and oxidative stress mole-cules.Results:12h after endotoxin injection,the water content of lung tissue in Rum group was(6.82 ± 0.97)%.After vari-ance analysis,the water content of lung tissue in ALI group was significantly higher than that in NC group,AQP1 and AQP5 protein levels in lung tissue were significantly lower than those of NC group,AQP3 and AQP4 protein levels were not different from those of NC group,and MPO,NF-kB,TNF-α,HMGB1,IL-8,ROS,ATP,MDA,Bax and Caspase-3 protein levels were significantly higher than those of NC group;the water content of lung tissue in Rum group was significantly lower than that in ALI group,AQP1 and AQP5 protein levels in lung tissue were significantly higher than those of ALI group,AQP3 and AQP4 protein levels were not different from those of ALI group,and MPO,NF-kB,TNF-α,HMGB1,IL-8,ROS,ATP, MDA,Bax and Caspase-3 protein levels were significantly lower than those of ALI group.Conclusion:The n-butanol extract of Rumex gmelini Turcz reduce the pulmonary edema,inflammatory response,oxidative stress and apoptosis during the endotox-in-induced ALI in mice.关键词
毛脉酸模/内毒素/急性肺损伤/炎症反应/氧化应激Key words
Rumex gmelini Turcz/endotoxin/acute lung injury/inflammatory response/oxidative stress分类
医药卫生引用本文复制引用
欧燕,商思阳,戴艺..毛脉酸模正丁醇部位对内毒素所致小鼠急性肺损伤的影响[J].海南医学院学报,2017,23(24):3331-3334,4.基金项目
重庆市科技厅民生科研基金(120990)Chongqing Municipal Science and Technology Department of the people's livelihood Research Fund(120990) （120990）
