华北农学报2017,Vol.32Issue(5):106-111,6.DOI:10.7668/hbnxb.2017.05.016
基于CSSL的水稻芽期耐盐性QTL定位
Mapping of QTLs for Bud-stage Salinity Tolerance Based on Chromosome Segment Substitution Line in Rice
摘要
Abstract
The identification of salt tolerant genetic loci in rice can provide study basis for the molecular mech-anism of salt tolerance,and gene resources for improving salt tolerant cultivars. A set of chromosome segment substi-tution lines ( CSSLs) developed from two sequenced rice cultivars:the indica variety 9311 as the recipient and the japonica variety Nipponbare as a donor were used to detect for salt tolerance in bud stage quickly,and to locate QTLs related to salt tolerance. 9 CSSLs showed significant salt tolerance and 4 QTLs were mapped by genetic back-ground detection based on high density molecular markers and the substitution mapping. These 4 QTLs located on rice chromosomes 1,2,4 and 11 chromosomes,designated as qSAT1,qSAT2,qSAT4 and qSAT11. qSAT1 was detec-ted in 4 CSSLs containing over lapping fragments,while the other 3 QTLs were detected in 2 CSSLs. These QTLs were not included in the same chromosome interval as the cloned rice salt tolerance genes,and thus were described as new candidate gene loci associated with bud-stage salt tolerance. The results supplies important information for further exploration and utilization of new salt tolerant QTL in rice.关键词
水稻/芽期/耐盐/QTLKey words
Oryza sativa L./Bud stage/Salt tolerance/QTL分类
农业科技引用本文复制引用
赵春芳,张善磊,赵庆勇,周丽慧,赵凌,姚姝,张亚东,王才林..基于CSSL的水稻芽期耐盐性QTL定位[J].华北农学报,2017,32(5):106-111,6.基金项目
国家科技支撑计划项目(2015BAD01B02) (2015BAD01B02)
江苏省重点研发项目(BE2016370) (BE2016370)
现代农业产业技术体系建设专项基金项目(CARS-01-62) (CARS-01-62)