华北农学报2017，Vol.32Issue(5)：112-116,5.DOI:10.7668/hbnxb.2017.05.017
甜菜双向电泳体系条件的优化
Optimization of Two Dimensional Electrophoresis System of Sugar Beet
摘要
Abstract
In order to obtain the best extraction method of protein in two-dimensional electrophoresis of beet leaves and establish the best two-dimensional electrophoresis system. It took beet leaves as material. To study the effects of the extraction methods of total protein,the pH value of IPG strip,and the corresponding amount of sample on the results of two-dimensional electrophoresis of sugar beet leaves through comparative analysis. The results showed that TCA/acetone extraction method was better than the phenol extraction method and soluble protein ex-traction method. Comparing IPG strip of pH 4-7 with pH 5-8 in two-dimensional electrophoresis:Protein spots with pH 4-7 were clear and distributed uniform in the spectra,no protein concentrated. It was more suitable for proteomics analysis of sugar beet leaves. Using the 7 cm linear phase IPG strip,protein spots with 150 μg sample volume increased obviously and was better. Using the 17 cm strip of pH 4-7 with 300 μg sample volume protein spots were more clear. The study provided the theoretical basis and reference for the study of sugar beet proteomics.关键词
甜菜/蛋白质提取方法/双向电泳/体系优化Key words
Sugar beet/Protein extraction method/Two-dimensional electrophoresis/System optimization分类
农业科技引用本文复制引用
张辉,王良,白晨,王华忠,张惠忠,李晓东,付增娟,赵尚敏,鄂圆圆,张自强..甜菜双向电泳体系条件的优化[J].华北农学报,2017,32(5):112-116,5.基金项目
内蒙古自治区自然科学基金项目(2016MS0374) （2016MS0374）
