华北农学报2017，Vol.32Issue(5)：117-123,7.DOI:10.7668/hbnxb.2017.05.018
二倍体马铃薯SSR遗传图谱的构建
Construction of an SSR-based Genetic Linkage Map of Diploid Potato
摘要
Abstract
In order to enrich molecular markers and position the bacterial wilt resistant gene loci in potato,an SSR-based genetic linkage map was constructed from a diploid population consisting of 123 individuals derived from a cross between two potato diploid clones ( C and E) which harborer resistance to Ralstonia solanacearum in this study. A total of 864 simple sequence repeat primer pairs were designed according to the potato genome sequence and used to screen parents C and E for polymorphisms. Of 187 polymorphic loci,72. 2％ showed clear amplifiable and scorable bands in the F1 population. Then a total of 135 locus were mapped to 12 linkage groups,of which the cumulative length was 948. 4 cM,with an average marker interval of 7. 03 cM,and 5 segregation distortion regions in three linkage groups. This study provided a set of new SSR markers for genetic map construction and increased satu-ration of map in potato. The relatively high density map may provide a useful reference for further identification, mapping of quantitative trait loci ( QTL) and cloning of potato bacterial wilt resistance genes and marker-assisted breeding.关键词
二倍体/马铃薯/SSR/连锁图谱Key words
Diploid/Potato/Simple sequence repeat/Linkage map分类
农业科技引用本文复制引用
朱云,郭晓,孙小磊,马伟清,董道峰,郭宝太,杨煜,杨晓慧,李广存..二倍体马铃薯SSR遗传图谱的构建[J].华北农学报,2017,32(5):117-123,7.基金项目
山东省自然科学基金项目(ZR2014YL014 （）
ZR2016CM27) （）
山东省农业科学院青年科研基金项目(2014QNZ03) （2014QNZ03）
