华北农学报2017，Vol.32Issue(5)：149-153,5.DOI:10.7668/hbnxb.2017.05.023
6-BA对葡萄果实中有机酸积累及相关基因表达的影响
Effect of 6-BA on Organic Acid Content and Related Genes Expression in Grape Berry
摘要
Abstract
In order to study the effect of 6-BA on grape fruit organic acid accumulation,and the possible mo-lecular mechanism of 6-BA regulating organic acid synthesis, a grape cultivar, Rizamat was selected to study the effect of 6-BA on fruit organic acid accumulation and related genes expression. The inflorescences and fruit clusters were treated with 6-BA at 5 days before blooming,3 and 10 days after anthesis respectively. The results showed that organic acid content of the mature grape fruit was closely related to treatment concentration of 6-BA. Tartaric acid and total organic acid content of the mature fruits that treated by 30 mg/L 6-BA was significantly lower than control. But it didn′t play important roles in the content of malic acid,citric acid and oxalic acid. For 10,20 mg/L 6-BA treatment,all kinds of organic acid didn′t show significant difference between treated and control mature fruits. Gene expression result showed that the expression of IDH,MDH and PEPC were mainly suppressed by 6-BA treatment,but it also promoted the expression of ME. Therefore,we speculated that the reason for inhibiting organic acid synthesis was that parts of the organic synthesis related genes expression were affected by 6-BA treatment.关键词
葡萄/6-BA/有机酸/基因表达Key words
Grape/6-BA/Organic acid/Gene expression分类
农业科技引用本文复制引用
王西成,钱亚明,吴伟民,赵密珍,周蓓蓓,王壮伟,巫建华..6-BA对葡萄果实中有机酸积累及相关基因表达的影响[J].华北农学报,2017,32(5):149-153,5.基金项目
江苏省自然科学基金项目(BK20160587) （BK20160587）
