华北农学报2017，Vol.32Issue(5)：163-170,8.DOI:10.7668/hbnxb.2017.05.025
外源NO协同N-乙酰半胱氨酸对肥城桃果实线粒体抗氧化系统的调控作用
Regulation by Exogenous Nitric Oxide and N-acetyl-L-cysteine on Mitochondrial Antioxidant System in Feicheng Peaches
摘要
Abstract
To study the regulation by exogenous nitric oxide ( NO ) and N-acetyl-L-cysteine ( NAC ) on mito-chondrial antioxidative system of Feicheng peaches during cold storage. Feicheng peaches were dipped in 15 μmol/L NO,80 mmol/L N-acetyl-L-cysteine ( NAC,reactive oxygen species scavenger) plus 15 μmol/L NO, and 5 μmol/L c-PTIO ( NO scavenger) solution,respectively. The changes in mitochondrial respiration,mitochon-drial membrane potential,the contents of malonaldehyde ( MDA) and reactive oxygen species ( ROS) and the activ-ities of antioxidant enzymes were detected. Treatment with 15 μmol/L NO significantly decreased mitochondrial ROS content. Especially at the 1st week,mitochondrial ROS content in peaches treated with 15μmol/L NO was on-ly 50％ of the control. Mitochondrial ROS content in peaches treated with 80 mmol/L NAC-15μmol/L NO was sig-nificantly lower than that of the control and other treatments after 2 weeks,while treatment with 5 μmol/L c-PTIO increased the content of mitochondrial ROS. The activities of antioxidative enzymes ( POD,SOD and CAT) in mito-chondria of peaches treated with 15 μmol/L NO was higher than that of the control and treatment with 5 μmol/L c-PTIO. Treatment with 80 mmol/L NAC-15 μmol/L NO increased the activity of mitochondrial SOD and decreased the activities of mitochondrial POD and CAT. Treatment with 5 μmol/L c-PTIO decreased the activities of antioxi-dative enzymes in mitochondria. Mitochondrial MDA content in peaches treated with 15μmol/L NO was lower than that of the control and other treatments during the storage except for the second week. However,mitochondrial MDA content in peaches treated with 80 mmol/L NAC-15μmol/L NO was significantly higher than that of the control and other treatments after 1 week. Mitochondrial MDA content in peaches treated with 5 μmol/L c-PTIO was higher than that of the control and treatment with 15 μmol/L NO in the second and third week. Mitochondrial membrane potential was improved by treatment with 15 μmol/L NO,reached the maximum,which was 1. 2 times as high as that of the control, at 3 weeks. Mitochondrial membrane potential of peaches treated with 80 mmol/L NAC-15μmol/L NO was higher than that of the control and other treatments. However,treatment with 5μmol/L c-PTIO de-creased mitochondrial membrane potential. The mitochondrial respiration of peaches was inhibited by treatment with 15 μmol/L NO. Mitochondrial respiration peak of peaches treated with 80 mmol/L NAC-15 μmol/L NO appeared 1 week earlier than the control. Treatment with 5 μmol/L c-PTIO maintained high respiration of mitochondria. Ex-ogenous nitric oxide and N-acetyl-L-cysteine could improve the antioxidant capacity of mitochondrial antioxidant system of peaches during storage,and delay the decrease of mitochondrial function.关键词
桃/NO/N-乙酰半胱氨酸/线粒体/抗氧化系统Key words
Peach ( Prunus persica)/Nitric oxide/N-acetyl-L-cysteine/Mitochondria/Antioxidant system分类
农业科技引用本文复制引用
高珊,王泉,高吉刚,朱树华..外源NO协同N-乙酰半胱氨酸对肥城桃果实线粒体抗氧化系统的调控作用[J].华北农学报,2017,32(5):163-170,8.基金项目
国家自然科学基金项目(31270723) （31270723）
