华北农学报2017，Vol.32Issue(6)：31-36,6.DOI:10.7668/hbnxb.2017.06.005
高油酸油菜脂肪酸代谢的蛋白质组学与转录组学关联分析
Transcriptomics and Proteomics Correlation Analysis of Fatty Acid Metabolism in High Oil Rape
摘要
Abstract
In order to study the regulation mechanism of fatty acid metabolism in high oleic rapeseed,a group of highly oleic acid near-isogenic lines was used as the material from 20 to 35 days after pollination,and was discussed by isobaric tags for relative and absolute quantitation with transcriptome technology. The results showed that the correlation coefficient between protein and transcript group was not high, and the correlation coefficient between quantitative protein and gene was-0 . 3269 . The correlation coefficient of differential protein and gene expression was-0. 7358,and the correlation coefficient of identical protein and gene expression was 0. 7143. Besides,80 dif-ferentially expressed genes were identified,GO annotation suggested that these genes involved in metabolism,signal transduction,defense and stress responses,redox,transcription,and so on,23 of the 80 genes were associated with fatty acid metabolism,16 of them were up-regulated and 7 down-regulated. gi|297330358(pantolactone ACP meth-yl ester) ,gi|297321940 ( phosphorylase) and gi|297314818 ( acetamide/formamidase family) may play an impor-tant role in the case of high oleic acid rapeseed fatty acid metabolism. This study shows that proteomics and tran-scriptase association analysis can provide a new idea for the mechanism of fatty acid metabolism in high oil rape.关键词
蛋白质组学/转录组学/关联分析/高油酸油菜/脂肪酸代谢Key words
Proteomics/Transcriptomics/Association analysis/High oleic rapeseed/Fatty acid metabolism分类
农业科技引用本文复制引用
王晓丹,肖钢,常涛,张振乾,官春云,邬贤梦..高油酸油菜脂肪酸代谢的蛋白质组学与转录组学关联分析[J].华北农学报,2017,32(6):31-36,6.基金项目
国家"973"计划项目(2015CB150206) （2015CB150206）
国家自然科学基金项目 (31201240) （31201240）
湖南省科技重大专项(2014FJ1006) （2014FJ1006）
作物种质创新与资源利用国家重点实验室培育基地开放课题 （）
湖南农业大学作物学开放基金项目(ZWKF201302) （ZWKF201302）
