华北农学报2017，Vol.32Issue(6)：37-44,8.DOI:10.7668/hbnxb.2017.06.006
药用野生稻ADF基因的克隆及其抗逆性分析
Cloning and Stress Resistance Analysis of ADF Gene in Oryza officinalis
摘要
Abstract
In order to investigate the effect of ADF gene on abiotic stress in Oryza officinalis. Based on the a-nalysis on the drought-stressed transcriptome of Oryza officinalis,OoADF1 was cloned by RT-PCR technique and se-quence analysis showed that OoADF1 contains 453 bp-length ORF and was intronless,and OoADF1 was predicted to contain 150 amino acids and have conserved ADF structure. Quantitative RT-PCR analysis showed that OoADF1 was expressed in all seven tissues, including leaf blade, leaf sheath and root at four-leaves stage, leaf blade, leaf sheath,stem and young panicle at heading stage in Oryza officinalis. The highest expression level of OoADF1 was detected in the root at four-leaves stage,while leaf sheath at heading stage. The fold of expression of OoADF1 a-mounted to 17. 9 and 40. 0 in the drought-or high NaCl-stressed young seedling,respectively. The coding sequence of OoADF1 was fused to the prokaroytic expression vector pET-28a( +), and the combinant vectors were induced by IPTG and detected by SDS-PAGE. The molecular weight of protein OoADF1 was detected to be 16 kDa,which was consistent with theoretical one. The results of stress tolerance assay showed that the recombinant strain produ-cing OoADF1 fusion protein improved the resistance to the high concentration,indicating that OoADF1 may exert a role in respond to salt stress and protect cells from high-salt harm. These results provides that OoADF1 has a certain degree of response to high salt stress,so that cells from high salt poisoning.关键词
药用野生稻/水稻/抗逆性/ADFKey words
Oryza officinalis/Rice/Resistance/ADF分类
生物科学引用本文复制引用
李培纲,李红梅,张帅,李劲松,陈志雄,刘向东..药用野生稻ADF基因的克隆及其抗逆性分析[J].华北农学报,2017,32(6):37-44,8.基金项目
国家自然科学基金项目(31271688) （31271688）
