华北农学报2017，Vol.32Issue(6)：54-59,6.DOI:10.7668/hbnxb.2017.06.008
棉花过表达超氧化物歧化酶基因对植株耐盐性的影响
Effects of Overexpression of Cotton Superoxide Dismutase Genes on Salt Tolerant Capability in Upland Cotton
摘要
Abstract
In order to explore the genetically modified effects of excessive expression of superoxide dismutase in the upland cotton. Cotton cytoplasmic superoxide dismutase gene sequences of nucleic acids was found in the Gen-bank database, a pair of specific primers were designed, the cotton cytoplasmic superoxide dismutase gene was cloned and the plant binary expression vector was built,the excessive expression of transgenic cotton plants were ob-tained by used a new technology of cotyledon axillary bud of Agrobacterium mediated genetic transformation. PCR and Southern Blot analysis indicated that the new copy Cu/Zn-SOD gene had been integrated into the genome of cotton. Germination experiment of genetically modified materials T1 generation seeds showed that under the stress of NaCl,the average length of taproot,bud average long and average number of lateral root of genetically modified ma-terials were greater than the negative control, the SOD activity in transgenic plants leaves were significantly im-proved than that in the control,so were the chlorophyll contents,the MDA content was dramatically declined,but praline contents was no changed. If excessive cytoplasmic SOD genes were expressed in the cotton,the SOD activity of plantlet can be increased,this can reduce salt stress caused by the cell membrane peroxidation,and enhance the ability of plants to resist salt stress.关键词
棉花/超氧化物歧化酶基因/遗传转化/耐盐能力Key words
Cotton/Superoxide dismutase gene/Genetic transformation/Salt tolerance ability分类
生物科学引用本文复制引用
胡根海,张晓红,付远志,董娜,王清连..棉花过表达超氧化物歧化酶基因对植株耐盐性的影响[J].华北农学报,2017,32(6):54-59,6.基金项目
河南省教育厅科学技术研究重点项目 (16A210023) （16A210023）
