化工进展2018，Vol.37Issue(1)：276-282,7.DOI:10.16085/j.issn.1000-6613.2017-0752
木薯酒精渣的预处理及补料同步糖化发酵制取乙醇
Pretreatment of cassava alcohol residues and ethanol production by simultaneous saccharification and fermentation
摘要
Abstract
Processing cassava alcohol residues(CAR)is one of the bottlenecks of the large scale application of the cassava based alcohol production. This study focused on the ethanol production using CAR as raw material by pretreatment of CAR and simultaneous saccharification and fermentation (SSF). The chemical compositions of cassava alcohol residues were analyzed. Pretreatment was conducted by different methods such as aqueous ammonia pretreatment,sodium hydroxide pretreatment and the combined pretreatment with aqueous ammonia and dilute sulfuric acid. The effect of different pretreatment methods on enzymatic digestibility of CAR were studied. The structural feature of CAR was analyzed by X-ray diffraction(XRD)and scanning electron microscope(SEM). The fed-batch method was combined with simultaneous saccharification and fermentation(SSF)to enhance ethanol concentration further and reduce enzyme loading. The results showed that among the three pretreatment methods the combined pretreatment was more effective on increasing cellulose and digestibility of cellulose. Cellulose increased 111.26% and lignin decreased 35.05% after being pretreated by the combined pretreatment. Compared with no pretreated cassava alcohol residues, cellulose conversion rate increased from 42.10% to 61.71% at 72h of enzyme hydrolysis when the CAR pretreated by combined pretreatment were used as substrate. After pretreatment by aqueous ammonia,the lignin decreased,the cellulose and crystallinity increased,and the surface of CAR became rougher. The ethanol concentration was 51.0g/L at 120h by fed-batch combined with SSF when the initial substrate concentration was 100.0g/L and the final substrate concentration of 400.0g/L by adding aqueous ammonia pretreated CAR at 20h,40h and 60h,respectively.关键词
燃料乙醇/木薯酒精渣/同步糖化发酵/预处理/纤维素结晶度Key words
fuel ethanol/cassava alcohol residues/simultaneous saccharification fermentation(SSF)/pretreatment/cellulose crystallinity分类
农业科技引用本文复制引用
岳军,徐友海,王继艳,胡世洋,惠继星,金刚..木薯酒精渣的预处理及补料同步糖化发酵制取乙醇[J].化工进展,2018,37(1):276-282,7.基金项目
中国石油科技管理部项目(2012A-1904-01)及吉林市战略新兴产业重大科技成果产业化计划(2015313008)项目. （2012A-1904-01）
