华南农业大学学报2018，Vol.39Issue(1)：14-19,6.
重离子诱发的2个水稻突变体表型鉴定及遗传分析
Phenotypic identification and genetic analysis of two rice (Oryza sativa L.) mutants induced by heavy ion
摘要
Abstract
[Objective] To clarify the phenotypic characteristics and genetic patterns of two rice (Oryza sativa L.) mutants.[Method] Two mutants were screened from the M2 generation of theindica rice germplasm BBS (wild type) induced by heavy ion, named m2 and m3 respectively. Firstly, the mutant materials were identified by means of phenotypic observation and agronomic trait comparison. Secondly, the F2 populations of 02428 (male parent)/m2 and 02428/m3 were constructed for genetic analysis.[Result] Compared with BBS, the leaves of m2 were inner-rolled and the leaf width was significantly reduced during the whole growth period. The rolling index of flag leaf, the 2nd and 3rd leaf from the top were 22.30％, 38.15％ and 28.84％ respectively, which were significantly different from BBS. The mutantm2 showed a high level of sterility. The new leaf tip of m3 main stem started necrosis on the 54th day after sowing in early season or 30th day after sowing in late season. The leaf necrosis phenotype sustained about 25 days and then new leaves recovered to normal phenotype. Compared with BBS, the main panicle weight and grain number of m3 dropped highly significantly, but other agronomic traits had no significant difference. Genetic analysis indicated that the frequency distribution of flag leaf width of the F2 population of m2/02428 was in accordance with normal distribution. The segregation ratio of normal individuals and leaf necrotic individuals fitted 3:1 in the F2 generation ofm3/02428.[Conclusion]The mutant m2 is a narrow leaf mutant and the narrow leaf trait is controlled by multiple genes, whereas m3 is a leaf necrosis mutant and the mutant phenotype is controlled by a pair of recessive nuclear genes.关键词
水稻/重离子诱变/突变体/窄叶/叶片枯萎Key words
rice (Oryza sativa L.)/heavy ion irradiation/mutant/narrow leaf/leaf necrosis分类
农业科技引用本文复制引用
彭歆,罗立新,张力,熊子墨,王慧,郭涛,陈志强,肖武名..重离子诱发的2个水稻突变体表型鉴定及遗传分析[J].华南农业大学学报,2018,39(1):14-19,6.基金项目
国家重点研发计划(2016YFD0102102) （2016YFD0102102）
广东省自然科学基金(2016A030313391) （2016A030313391）
现代农业产业技术体系建设专项资金(CARS-01-12) （CARS-01-12）
