华南农业大学学报2018，Vol.39Issue(1)：33-41,9.
不同灌溉方式和尿素猪粪比例对稻田氮素转化相关微生物活性的影响
Effects of different irrigation methods and ratios of urea pig manure on microbial activity related to nitrogen transformation in paddy soil
摘要
Abstract
[Objective]To understand the changes of microbial indexes related to nitrogen transformation in paddy field under "thin-shallow-wet-dry" and alternate drying and wetting irrigation methods.[Method]We used three irrigation methods (CIR: conventional irrigation, TIR: "thin-shallow-wet-dry" irrigation, DIR: alternate drying and wetting irrigation), and three nitrogen (N) treatments (FM1: all urea, FM2: 30％ urea substituted by pig manure, FM3: 50％ urea substituted by pig manure). The numbers of nitrite bacteria, nitrate bacteria, denitrifying bacteria and the activities of urease, hydroxylamine reductase, nitrite reductase and nitrate reductase were measured at tillering, booting, milky and ripening stages. The relationships among the microbial activity indices were analyzed.[Result]At booting stage, the numbers of nitrite bacteria, nitrate bacteria and denitrifying bacteria were relatively large, and the activities of urease, nitrite reductase and nitrate reductase were relatively high in soil under different treatments. Hydroxylamine reductase activity was relatively high by DIR method. Under FM3 treatment, compared to CIR method, DIR method increased the number of nitrite bacteria by 2.31 times at tillering stage, increased the number of nitrate bacteria and the activities of urease, hydroxylamine reductase and nitrite reductase by 2.07, 0.81, 554.72 and 1.78 times from tillering stage to milky stage, but reduced the average number of denitrifying bacteria and nitrate reductase activity by 31.34％ and 43.82％ respectively at four growth stages. The differences of microbial indexes related to nitrogen transformation in paddy soil between TIR and CIR methods varied with N treatments and growth stages. Using DIR method, compared to FM1, FM3 significantly increased the number of nitrite bacteria at milky stage, the number of nitrate bacteria at booting and ripening stages, the number of denitrifying bacteria at tillering and ripening stages, urease activity at milky and ripening stages, hydroxylamine reductase activity from tillering stage to milky stage, and activities of nitrite reductase and nitrate reductase at booting stage. Except the insignificant correlations among nitrate reductase activity, the number of nitrate bacteria and nitrite reductase activity, there were significant correlations among other microbial indexes.[Conclusion]The rice booting stage is the active stage of microbial activity related to nitrogen transformation in paddy field. DIR method can effectively enhance most microbial indexes related to nitrogen transformation in soil at tillering and booting stages. TIR method and FM3 treatment can significantly increase microbial activities related to nitrogen transformation in soil at milky and ripening stages.关键词
节水灌溉/有机无机氮比例/水稻土/氮素转化/酶活性Key words
water-saving irrigation/ratio of organic to inorganic nitrogen/paddy soil/nitrogen transformation/enzyme activity分类
农业科技引用本文复制引用
陈意超,李伏生,李烙布..不同灌溉方式和尿素猪粪比例对稻田氮素转化相关微生物活性的影响[J].华南农业大学学报,2018,39(1):33-41,9.基金项目
国家自然科学基金(51469003) （51469003）
