华南农业大学学报2018，Vol.39Issue(1)：59-65,7.
内源激素对同源四倍体黑皮冬瓜自交花粉萌发生长的影响
Effects of endogenous hormones on pollen germination and growth of autotetraploid black wax gourd
摘要
Abstract
[Objective]To study the relationship between pollen germination and growth of autotetraploid Benincasa hispida (Thunb.) Cogn. and endogenous hormones, and explore new ideas for understanding the low fertility mechanism of autotetraploid B. hispida.[Method]Taking autotetraploid and diploid B. hispida as materials, germination and growth of self-pollinated pollen in pistil and the contents of endogenous hormones in pollens and pistils were studied.[Result]The germination rate of self-pollinated pollen of autotetraploid B. hispida was low on the stigma. Pollen tube growth was slow. There were some abnormal phenomenons during growth of pollen tubes, such as uneven thickness, nodule formation, swelling at the tip, two pollen tubes in one pollen, distorted and folded pollen tubes. Compared with diploid B. hispida, the pistils and pollens of autotetraploid B. hispida had significantly lower zeatin riboside (ZR) and isopentenyl adenosine (IPA) contents (P<0.01), significantly lower gibberellin A3 (GA3) content (P<0.05) in pistils, silightly lower gibberellin A3 (GA3) content (P>0.05) in pollens, slightly lower indole-3-acetic acid (IAA) content (P>0.05), significantly higher abscisic acid (ABA) content (P<0.05), and significantly lower ratio of (IAA+GA3+ZR+IPA) to ABA contents (P<0.01).[Conclusion]The endogenous hormones in the pistils and pollens of autotetraploid B. hispida regulate pollen germination and growth and cause abnormal phenomenons in pollen tubes, which is one of the reasons for the low fertility of autotetraploid B. hispida.关键词
黑皮冬瓜/内源激素/同源四倍体/花粉/花粉管/雌蕊Key words
Benincasa hispida (Thunb.) Cogn./endogenous hormone/autotetraploid/pollen/pollen tube/pistil分类
农业科技引用本文复制引用
万正林,周艳霞,邓俭英,李立志,武鹏,龙明华..内源激素对同源四倍体黑皮冬瓜自交花粉萌发生长的影响[J].华南农业大学学报,2018,39(1):59-65,7.基金项目
广西科学研究与技术开发计划项目(桂科攻1222009-3D) （桂科攻1222009-3D）
国家现代农业产业技术体系广西大宗蔬菜创新团队专项资金(nycytxgxcxtd-03-10-1) （nycytxgxcxtd-03-10-1）
广西科技基地和人才专项(桂科AD17129042) （桂科AD17129042）
广西农业科学院科技发展基金项目(桂农科2012JZ12) （桂农科2012JZ12）
