华南农业大学学报2018，Vol.39Issue(1)：66-71,6.
抑制miR-29a-3p表达对小鼠巨噬细胞凋亡的影响及机制
Mechanism and influence of down-regulation ofmiR-29a-3p expression on apoptosis in mouse macrophage
摘要
Abstract
[Objective]To study the influence of down-regulation of miR-29a-3p expression on apoptosis in mouse macrophage and expression of apoptosis-associated genes, and provide references for investigating the pathogenesis mechanism of mycobacterium tuberculosis (MTB) and developing new diagnosis and treatment methods.[Method]The recombinant inhibitor vector pEZX-AM02-miR-29a-3p was transfected into RAW264.7 cells. Transfection efficiency was observed through fluorescence microscopy, the expression levels of miR-29a-3p and apoptosis-associated genes caspase3,caspase7,caspase8,Bcl-2,Mcl-1 and Bax were detected by RT-PCR, and the apoptosis rates of RAW264.7 cells were detected by flow cytometry at 24, 36, 48 h after transfection.[Result]After transfected with pEZX-AM02-miR-29a-3p recombinant inhibitor vector, miR-29a-3p expression in RAW264.7 cells decreased significantly, while the target genescaspase7,caspase8, Bcl-2,Mcl-1 and Bax were up-regulated at different levels. The results of flow cytometry indicated that the apoptosis rates increased as the transfection time progressed and reached the peak at 36 h.[Conclusion]The expression of miR-29a-3p in mouse macrophage are inhibited by transfecting with pEZX-AM02-miR-29a-3p, which promotes the apoptosis of macrophage by up-regulating the expression of target genes, suah ascaspase7, caspase8,Bcl-2 and Mcl-1.关键词
miR-29a-3p/抑制载体/巨噬细胞/细胞凋亡/靶基因Key words
miR-29a-3p/inhibitor vector/macrophage/cell apoptosis/target gene分类
农业科技引用本文复制引用
李木楠,李双双,孟凡爽,韩鹏,张文慧,张林波..抑制miR-29a-3p表达对小鼠巨噬细胞凋亡的影响及机制[J].华南农业大学学报,2018,39(1):66-71,6.基金项目
吉林省科技发展计划项目(20130101105JC, 20140204018YY) （20130101105JC, 20140204018YY）
吉林省教育厅"十三五"科学技术项目(2016-177JJKH20170304KJ) （2016-177JJKH20170304KJ）
吉林农业大学博士科研启动基金(2015015) （2015015）
