华南农业大学学报2018，Vol.39Issue(1)：78-84,7.
响应面法优化绿僵菌产绿僵菌素A的培养条件
Optimization of culture condition of Metarhizium anisopliae for destruxin A production by response surface methodology
摘要
Abstract
[Objective]To raise the destruxin A production of Metarhizium anisopliae by optimizing culture conditions of liquid fermentation.[Method]Experiments were designed by response surface methodology (RSM). The key factors affecting the liquid fermentation were screened by Plackett-Burman design and central composite design.[Result]A model with high fitting degree and small error was obtained. The optimum culture condition of this model was 22.7 g·L-1 sucrose, 13.4 g·L-1 peptone and 9.70 d for fermentation. The maximum predicted concentration of destruxin A was 6.90 μg·mL-1, while the actual measured concentration was 6.89 μg·mL-1.[Conclusion]The results can provide a theoretical basis for large-scale fermentation of M. anisopliae and production of crude toxin, which can potentially enable the widespread development and application of crude toxin.关键词
绿僵菌/绿僵菌素/产量/响应面法/液体发酵/培养条件Key words
Metarhizium anisopliae/destruxin/yield/response surface methodology/liquid fermentation/culture condition分类
农业科技引用本文复制引用
张功营,杨华,董丽红,万树青..响应面法优化绿僵菌产绿僵菌素A的培养条件[J].华南农业大学学报,2018,39(1):78-84,7.基金项目
广东省烟草专卖局(公司)科技项目(粤烟科[2015]7号) （公司）
