河南农业科学2017，Vol.46Issue(10)：137-142,6.DOI:10.15933/j.cnki.1004-3268.2017.10.025
鸡源肠炎沙门氏菌快速检测方法的建立及其分离株的耐药性分析
Establishment of Rapid Detection Method of Salmonella enteritidis and Drug Resistance Analysis of Isolates from Chickens
摘要
Abstract
To further investigate the mechanism of antibiotic resistance of Salmonella enteritidis (S.enteritidis),the specific primers were designed,based on sdf Ⅰ of S.enteritidis from the GenBank.A PCR method for S.enteritidis detection was developed after optimizing reaction conditions,and used for detection of clinical S.enteritidis isolated from chickens.The isolates were evaluated for antimicrobial sensitivity by K-B disc diffusion method against 22 antimicrobial drugs and the resistance genes of the isolates were detected by PCR.Electrophoresis showed that 203 bp fragment was detected,and the PCR assay could detect 100 pg/μL of DNA sensitivity,and 4 × 103 cfu/mL of the bacteria sensitivity,respectively.Compared with the traditional serological assay,the coincidence rate of the PCR for the isolates were 100％.The results of drug sensitive test showed that 56 isolates were resistance to the 22 antimicrobial agents in some degree,and penicillin (98.21％) and erythromycin (96.43％) were the highest.Test of resistance genes exhibited that the detectable rate of gene tetA,aph (3')-Ⅱ a,blaCMY-2,cat1 and sul1 was higher(more than 50％).In conclusion,the developed PCR assay was more specific,sensitive and rapid for the detection of S.enteritidis,and was able to be used for the rapid identification of S.enteritidis and epidemiological investigation.The resistance analysis results showed that the problem of resistance was serious,and resistance genes were widely existed in the resistant strains.关键词
鸡/肠炎沙门氏菌/检测/药敏试验/耐药基因Key words
chicken/Salmonella enteritidis/detection/drug sensitive test/resistance gene分类
农业科技引用本文复制引用
范忠军,许保疆,翟崇凯,郁川,王欢莉..鸡源肠炎沙门氏菌快速检测方法的建立及其分离株的耐药性分析[J].河南农业科学,2017,46(10):137-142,6.基金项目
盐城市农业科技指导性计划项目(YKN2013014) （YKN2013014）
盐城师范学院博士科研基金项目(12YSYJB0116) （12YSYJB0116）
