解剖学杂志2017，Vol.40Issue(6)：651-655,5.DOI:10.3969/j.issn.1001-1633.2017.06.002
过氧化物酶Ⅰ、过氧化氢酶、超氧化物歧化酶在大鼠肝缺血再灌注损伤小肠内的表达变化
Expression changes of peroxiredoxin, catalase and superoxide dismutase in the small intestine of rats with hepatic ischemia reperfusion injury
牛波 1王切 2刘风藏 2王磊2
作者信息
- 1. 河北省儿童医院,石家庄050030
- 2. 河北医科大学解剖学教研室,石家庄050017
- 折叠
摘要
Abstract
Objective: To observe expression change of peroxiredoxin, catalases and superoxide dismutase in the small intestine of rats with hepatic ischemia reperfusion injury, and level of oxidative stress in the small intestine. Methods: The hepatic blood vessel and bile duct leading to both the left and middle hepatic lobes were first clipped and then released thirty minutes later to restore the blood supply so as to establish 70% hepatic ischemia reperfusion injury model of rats. The serum, liver and small intestine tissues were taken 6 hours after the reperfusion. The activities of alanine aminotransferase (ALT) in the serum were detected with full automatic biochemical aualyzer. The malondialdehyde contcnt in the serum and small intestine tissues were determined with thiobarbituric acid colorimetric method. The H2O2 content in the serum and small intestine tissues were detected with spectrophotometric method. The morphological changes of the liver and small intestine were observed under microscope using H-E staining. The expression of peroxiredoxin, catalase and superoxide dismutase mRNA in small intestine tissues was evaluated by RT-PCR and protein level of peroxiredoxin, catalase and superoxide dismutase was estimated by Western blotting. Results: Compared with the control group, the ALT activity and the content of malondialdehyde and H2O2 in the serum of rats with hepatic ischemia reperfusion injury were increased significantly. H-E staing results of hepatic ischemia reperfusion injury group showed that the morphology of the liver was significantly impaired. The content of malondialdehyde and H2O2 in small intestine tissues of the hepatic ischemia reperfusion injury group was higher than that of the control group; the mRNA and protein level of peroxiredoxin, catalase and superoxide dismutase in the small intestine were also increased significantly. Conclusion: Peroxiredoxin, catalase and superoxide dismutase may play a role of anti-oxidative stress when the small intestine is in a state of high oxidative stress.关键词
肝/缺血再灌注损伤/过氧化物酶Ⅰ/过氧化氢酶/超氧化物歧化酶/氧化应激/大鼠Key words
liver/ischemia reperfusion injury/peroxiredoxin Ⅰ/catalase/superoxide dismutase/oxidative stress/rat引用本文复制引用
牛波,王切,刘风藏,王磊..过氧化物酶Ⅰ、过氧化氢酶、超氧化物歧化酶在大鼠肝缺血再灌注损伤小肠内的表达变化[J].解剖学杂志,2017,40(6):651-655,5.
