解剖学杂志2017，Vol.40Issue(6)：682-684,734,4.DOI:10.3969/j.issn.1001-1633.2017.06.009
不同浓度红景天苷对Ⅱ型糖尿病大鼠骨骼肌磷脂酰肌醇-3-激酶及葡萄糖转运蛋白-4表达的影响
Effects of different concentrations of salidroside on expression of PI3K and GLUT4 in skeletal muscle of type Ⅱ diabetic rats
摘要
Abstract
Objective: To explore the mechanism of salidroside in improving insulin resistance in the skeletal muscle. Methods:The type Ⅱ diabetic rat model was established with a small dose of STZ plus high-fat and high calorie feeding. The modeled rats were randomly divided into diabetic model (DM) group, salidroside low-dose group, salidroside middle-dose group, salidroside high-dose group and metformin group. Another 10 rats were set as normal control group (NC). Blood glucose, blood lipid and insulin levels were measured after 12 weeks. The rats were sacrificed and the hind legs were removed. Western blotting was used to detect the expression levels of PI3K and GLUT4 in tissues. Results: Compared with NC group, the expression of PI3K and GLUT4 in skeletal muscle cells of rats in DM group was significantly reduced. Compared with DM group, the expression of PI3K and GLUT4 in skeletal muscle cells was significantly enhanced in drug treatment group. Conclusion: Salidroside may improve insulin resistance in type Ⅱ diabetic rats by increasing the expression of PI3K and GLUT4 in rat skeletal muscle cells.关键词
Ⅱ型糖尿病/胰岛素抵抗/磷脂酰肌醇-3-激酶/葡萄糖转运蛋白-4/红景天苷Key words
type Ⅱ diabetes mellitus/insulin resistance/phosphatidylinositol-3-kinase/glucose transporter-4/salidroside引用本文复制引用
高静媛,杨雨旸,杜晨光,邢磊,李继安,包巨太..不同浓度红景天苷对Ⅱ型糖尿病大鼠骨骼肌磷脂酰肌醇-3-激酶及葡萄糖转运蛋白-4表达的影响[J].解剖学杂志,2017,40(6):682-684,734,4.基金项目
河北省卫生厅项目(20130062) （20130062）
