军事医学2017，Vol.41Issue(9)：723-726,4.DOI:10.7644/j.issn.1674-9960.2017.09.005
冰冻血小板透析式洗涤方法研究
Protocol of dialysis-type processing on frozen platelets
曹卉 1易晓阳 1刘敏霞 1王建云 1王捷熙 1骆群 2韩颖1
作者信息
- 1. 军事医学科学院野战输血研究所,全军血液安全与保障重点实验室,血液安全保障技术研究北京市重点实验室,北京100850
- 2. 军事医学科学院附属医院输血科,北京 100071
- 折叠
摘要
Abstract
Objective To establish a new dialysis washing method for human frozen platelets and to evaluate a new type of washing machine based on dialysis.Methods Twenty bags of platelets(1U)were divided into two groups:centrifugation group and dialysis group.The effect of the washing machine was evaluated by measuring platelet function in vitro.Results After the platelets were treated by the two methods, the recovery rate of dialysis group[(73.57 ± 12.49)%]was significantly better than that of centrifugal group[(62.21 ±7.07)%].The osmotic pressure of dialysis group was 305.8 ±5.27mOsm/kg, compared to 383.2 ±22.00 mOsm/kg in centrifugation group.There was significant differences in the recovery rate and osmotic pressure(P<0.05), but there was no significant difference between dialysis group and centrifugation group in the mean platelet volume[(9.44 ±0.93)vs(9.23 ±0.50)fl], ADP-induced aggregation rate[(20.7 ±8.00)%vs(27.7 ±11.83)%],thrombin-induced platelet aggregation rate[(84.1 ±7.88)%vs(92.9 ±5.28)%], hypotonic shock responses[(19.02 ±4.42)% vs(23.19 ±9.08)%], CD62p activation rate [(16.00 ±9.40)%vs(21.86 ±8.14)%]or apoptosis rate[(46.31 ±10.28)% vs(54.59 ±16.76)%](P>0.05). Conclusion Compared to centrifugation group,the frozen platelet washing machine can not only improve platelet recovery,but also solve the problem of excessive DMSO residues.关键词
冰冻血小板/洗涤机/低温保存/质量评价Key words
frozen platelet/washing machine/cryopreservation/quality evaluation分类
医药卫生引用本文复制引用
曹卉,易晓阳,刘敏霞,王建云,王捷熙,骆群,韩颖..冰冻血小板透析式洗涤方法研究[J].军事医学,2017,41(9):723-726,4.
