军事医学2017，Vol.41Issue(9)：727-729,738,4.DOI:10.7644/j.issn.1674-9960.2017.09.006
冰冻红细胞洗涤技术优化及应用效果评价研究
Improved treatment of frozen red blood cells by red blood cell processing technology
刘敏霞 1韩颖 1王东根 1易晓阳 1王捷熙 1谷涛 2杜为 1周建伟 1王艳 1王建云 1曹卉1
作者信息
- 1. 军事医学科学院野战输血研究所,全军血液安全与保障重点实验室,血液安全保障技术研究北京市重点实验室,北京 100850
- 2. 四川南格尔医用股份有限公司,成都 610041
- 折叠
摘要
Abstract
Objective To improve the buffer for washing frozen red blood cells(RBCs)and explore the feasibility of replacing the middle buffer of 2% NaCl with 0.9% NaCl solution in the protocol of deglycerolization and the effect was evaluated.Methods Two units of RBCs separated from whole blood of healthy donors were frozen at -80℃.The thawed frozen RBCs were treated with a washing machine.On the basis of the old protocol(protocol 1),the middle buffer of 2%NaCl was replaced,and the usage of washing buffer and the washing steps were adjusted to create a new protocol(protocol 2).The quality of RBCs washed by the two protocols was evaluated.Results According to the results of the detection of RBCs treated by protocol 1 and 2: the hemoglobin contents(g)were 42.18 ±3.35 and 44.98 ±1.68,respectively,free hemoglobin contents(g/L)were 0.53 ±0.06 and 0.45 ±0.05 respectively, the residual amount of white blood cells (×107)was 1.92 ±1.04 and 1.12 ±1.12,and the osmolarities(mOsm)were 327.0 ±9.06 and 331.8 ±10.62 respectively. Sterile experiments were negative for both bacteria and fungi.The hemolysis rates(%)were 12.02 ±5.78 and 14.30 ± 5.67 respectively.The deforming abilities(%)were 21.42 ±1.45 and 21.32 ±0.84 respectively.The RBC recoveries (%)were 77.18 ±5.58 and 79.63 ±2.06 respectively.The processing time(min)was 79.60 ±0.55 and 78.80 ±1.30 respectively.There was no significant difference in the quality of frozen RBCs washed by the old protocol(protocol 1)and the new protocol(protocol 2)(P>0.05).Conclusion The quality of frozen RBCs washed by the two protocols meet national standards.关键词
冰冻红细胞/血细胞处理仪/程序优化/质量评价Key words
frozen RBC/blood cell washing instrument/program optimization/quality evaluation分类
医药卫生引用本文复制引用
刘敏霞,韩颖,王东根,易晓阳,王捷熙,谷涛,杜为,周建伟,王艳,王建云,曹卉..冰冻红细胞洗涤技术优化及应用效果评价研究[J].军事医学,2017,41(9):727-729,738,4.
