军事医学2017，Vol.41Issue(9)：730-734,741,6.DOI:10.7644/j.issn.1674-9960.2017.09.007
海上储运血液红细胞的能量代谢评价
Metabolism evaluation of blood quality after marine storage and transportation
王艳冰 1王璇琳 1谈文龙 1任素萍 1于群 1徐凤梅 2王钰 1房永青 3李常兰 1郐启源 1蒋兴伟 1高锋华 1贺敏1
作者信息
- 1. 军事医学科学院野战输血研究所,全军血液安全与保障重点实验室,血液安全保障技术研究北京市重点实验室,北京 100850
- 2. 安丘市市立医院,山东安丘 262100
- 3. 安丘市市场监督管理执法大队卫生监管组,山东安丘 262100
- 折叠
摘要
Abstract
Objective To evaluate the quality of blood after marine storage and transportation,to establish a quantitative energy metabolism platform supported by extracellular flux(XF)technique to determine the glycolysis level of red blood cells(RBC), and to introduce the "functional dose", as a valuable supplement, into the quality evaluation of RBCs after marine storage and transportation.Methods The extracellular acidification rate(ECAR)of erythrocyte glycolysis was detected by XF.The levels of glycolysis, ATP contents and free hemoglobin of RBCs were detected before and after marine navigation.RBC suspensions stored at 4℃in a blood bank on land served as control.Results The results showed that the glycolysis level of RBCs was detected by XF.Indicators of blood quality and energy metabolism of RBCs were detected in the twice marine storage and transportation.Blood samples transported around the Gulf of Bohai had no significant change,indicating that the samples were well preserved.The energy indicators of blood samples navigated in the Pacific Ocean decreased significantly,and it was recommended that the samples be discarded.Conclusion XF technique has been applied to the measurement of erythrocyte glycolysis,enabling high-throughput and multi-indicator dynamic detection of viable cells.The energy metabolism of erythrocyte should be recommended as an important indicator for evaluating blood after marine storage and transportation.关键词
船舶/XF技术/红细胞/运输/血液保存/能量代谢Key words
ships/XF technology/erythrocytes/transportation/blood preservation/energy metabolism分类
医药卫生引用本文复制引用
王艳冰,王璇琳,谈文龙,任素萍,于群,徐凤梅,王钰,房永青,李常兰,郐启源,蒋兴伟,高锋华,贺敏..海上储运血液红细胞的能量代谢评价[J].军事医学,2017,41(9):730-734,741,6.
