军事医学2017，Vol.41Issue(9)：762-766,5.DOI:10.7644/j.issn.1674-9960.2017.09.016
发光细菌在不同生理介质中增殖活性的可视化研究
Visualization of proliferation activity of luminescent bacteria in different physiological media
刘菁 1孙宇 2李慧 1何楚琳 3王茹 2李玲 1王会中 1王小慧1
作者信息
- 1. 解放军305医院,北京 100017
- 2. 军事医学科学院野战输血研究所,全军血液安全与保障重点实验室,血液安全保障技术研究北京市重点实验室,北京 100850
- 3. 解放军火箭军总医院,北京 100088
- 折叠
摘要
Abstract
Objective To investigate the relationship between the number of colonies and the luminous intensity of two kinds of platelets contamination bacteria,Escherichia coli(ECO)and Pseudomonas aeruginosa(PAE)in single platelets, plasma and phosphate buffered salines(PBS), and to explore their growth and proliferation in different biological fluid media.Methods The two luminescent bacteria were constructed before the correlations between the number of colonies and the luminous intensity in different media in vitro were observed with a small animal bioluminescence imaging instrument, and correlation analysis was carried out.By recording the luminous intensity at serial time points in different media, a growth curve was drown to reflect the proliferation of bacteria.Results There was a significant positive correlation between the number of colonies and luminous intensity of ECO and PAE in single platelets,plasma and PBS.The larger the number of colonies was,the stronger the luminous intensity was.The results indicated that single platelets significantly inhibited the proliferation of ECO and PAE,and that plasma also had some inhibitory effect,but not so strong.Conclusion For the two luminous bacteria,luminous intensity can represent the growth and proliferation of bacteria.Single platelets have obvious inhibitory effect on the growth of ECO and PAE, and the plasma also has some inhibitory effect, but the effect is not so strong as that of single platelets.关键词
发光细菌属/大肠杆菌/假单胞菌,铜绿/单采血小板/血浆/增殖Key words
Photobacterium/Escherichia coli/Pseudomonas aeruginosa/single platelets/plasma/proliferation分类
医药卫生引用本文复制引用
刘菁,孙宇,李慧,何楚琳,王茹,李玲,王会中,王小慧..发光细菌在不同生理介质中增殖活性的可视化研究[J].军事医学,2017,41(9):762-766,5.
