江西农业学报2018，Vol.30Issue(1)：7-12,26,7.DOI:10.19386/j.cnki.jxnyxb.2018.01.02
副猪嗜血杆菌临床分离菌的药物敏感性试验与ERIC-PCR基因分型
Antimicrobial Susceptibility Test and ERIC-PCR Genotyping of Haemophilus parasuis Isolates from Pigs in Seven Provinces
摘要
Abstract
One hundred and four strains of Haemophilus parasuis ( HPS) were isolated from 323 suspected pathological pig materials in seven provinces (Henan, Hubei, etc.). These HPS isolates were identified through 16s RNA PCR. The susceptibili-ties of these HPS isolates to 12 kinds of antibiotics were determined by broth microdilution method, and the genotyping of HPS i-solates was carried out by using ERIC-PCR. The study showed that all strains were sensitive to mequindox and valnemulin, and their resistance rate to trimethoprim-sulfamethoxazole, tetracycline, ciprofloxacin, lincomycin, kanamycin, thiamphenicol, eryth-romycin, ampicillin, ceftiofur and cefquinome was 93.3％, 51.9％, 51.0％, 26.0％, 10.6％, 13.5％, 37.5％, 39.4％, 5.8％and 1.9％, respectively. Most strains were resistant to 2～5 kinds of antibiotics, and their proportion was 69.2％. Among 104 HPS isolates, 99 isolates could be divided into 18 ERIC-PCR types according to 80％ similarity. Among all ERIC-PCR types, (XIII) type included the most strains (25 strains), which was followed by Ⅵ type (18 strains), Ⅷ type (13 strains), Ⅸ type (13 strains) and Ⅶ type (9 strains). Except the lincomycin-resistant isolates were almost equally distributed in 12 ERIC-PCR types, most of isolates resistant to other 5 kinds of antibiotics were concentratedly distributed in 2～3 ERIC-PCR types.关键词
副猪嗜血杆菌/分离鉴定/药物敏感性/ERIC-PCR/基因分型Key words
Haemophilus parasuis/Isolation and identification/Antimicrobial susceptibility/ERIC-PCR/Genotyping分类
农业科技引用本文复制引用
孙华润,翟亚军,蔡田,徐引弟,苑丽,潘玉善,刘建华,吴华,胡功政..副猪嗜血杆菌临床分离菌的药物敏感性试验与ERIC-PCR基因分型[J].江西农业学报,2018,30(1):7-12,26,7.基金项目
"十三五"国家重点研发计划(2016YFD0501304). （2016YFD0501304）
