检验医学与临床2018，Vol.15Issue(1)：4-8,5.DOI:10.3969/j.issn.1672-9455.2018.01.002
结核抗体蛋白芯片技术与TB-IGRA对结核病活动度及疗效的监测价值
The value of tuberculosis antibody protein chip technique and TB-IGRA in monitoring the activity and efficacy of tuberculosis
摘要
Abstract
Objective The aim of the study is to determine the value of protein chip technique and TB in-terferon gamma release test (TB-IGRA ) for the detection of tuberculosis activity and efficacy .Methods Ac-cording to lesion distribution ,a total of 655 patients with tuberculosis were divided into three groups :pulmo-nary tuberculosis group ,extrapulmonary tuberculosis group ,and pulmonary&extrapulmonary tuberculosis group ,with non-tuberculosis patients as the control group .Protein chip technique and TB-IGRA were adopted to compare the dynamic changes of the positive rates at different time points (prior treatment ,during therapeu-tic process ,6 and 12 months after end-of-treatment) in each group .The above results were compared and ana-lyzed with the results of bacteria-collecting smear method synchronously .Results The results showed that the positive rates of protein chip technique and TB-IGRA were decreased with the course of anti-tuberculous therapy .The positive rate of protein chip technique was lower than that of TB-IGRA during three-month ini-tial treatment period ,began to decline at the fourth month time-point ,and less than that of TB-IGRA ,then de-creased gradually .The positive rate was still respectively 18 .78％ and 15 .27％ at 6-month time-point and 12-month time-point after the end of treatment .The positive rate of TB-IGRA was evidently declined in initial treatment for 2 months ,and the decline rate was higher than that of protein chip technique .With regard to TB-IGRA ,the positive rate fell to 6 .26％ and 3 .82％ respectively at 8-month time-point and 12-month time-point in retreatment cases ,significantly lower than that of protein chip technique at the same time-points .With bac-teria-collecting smear method as the reference ,the diagnostic coincidence rate of protein chip technique was higher than that of TB-IGRA before initial treatment for 4 months ,decreased slightly at the fifth month time-point in initial treatment ,and then increased gradually with the progress of the treatment .Conclusion It is suggested that the protein chip technique and TB-IGRA have the value of monitoring the efficacy of tuberculo-sis ,and the value of protein chip technique is higher than that of TB-IGRA before initial treatment for 4 months ,and the value of protein chip technique is lower than that of TB-IGRA after the initial treatment for 4 months .Each of protein chip technique and TB-IGRA has its advantages and disadvantages ,and the combina-tion of the two assay methods has a good complementary effects .The clinical value of combined detection of protein chip technology and TB-IGRA has high clinical value in tuberculosis activity assessment and anti tu-berculosis effect monitoring .关键词
蛋白芯片/结核/γ-干扰素释放试验Key words
protein chip/tuberculosis/interferon gamma release assay分类
医药卫生引用本文复制引用
冯英凯,王勇,黄正谷,赵小莉,刘英..结核抗体蛋白芯片技术与TB-IGRA对结核病活动度及疗效的监测价值[J].检验医学与临床,2018,15(1):4-8,5.基金项目
重庆市卫生和计划生育委员会医学科研重点项目(2013-1-038). （2013-1-038）
