检验医学与临床2018，Vol.15Issue(1)：16-18,3.DOI:10.3969/j.issn.1672-9455.2018.01.005
精子自发顶体反应率与体外受精胚胎质量的相关性研究
Correlation between sperm spontaneous acrosome reaction and in vitro fertilization embryo quality
摘要
Abstract
Objective To investigate the correlation between spontaneous acrosome reaction of spermatozoa as well as two different methods to separate sperm and the quality of in vitro fertilization (IVF) embryos . Methods 200 male treated by IVF were randomly divided into observation group and control group (n=100) . Semen sample of the patients in the control group were treated with density gradient centrifugation ,and the patients in observation group were treated with density gradient centrifugation + swim-up methods .Two groups were receiving conventional IVF fertilization .The fertilization rate and the optimal embryo rate were compared between the two groups ,the correlation between sperm spontaneous acrosome reaction of the obser-vation group and IVF embryo quality .Results The fertilization rate of the observation group was significantly higher than that of the control group(P<0 .05) ,and the AR rate was significantly negatively correlated with the fertilization rate(r= -0 .890 ,P<0 .05) .The excellent embryo rate of the observation group was signifi-cantly higher than that of the control group (P<0 .05) ,and the AR rate was significantly negatively correlated with the optimal embryo rate(r= -0 .647 ,P<0 .05) .Conclusion Sperm spontaneous acrosome reaction rate which reduced by density gradient centrifugation +swim-up methods is helpful to improve the success rate of IVF and has clinical value in predicting the quality of IVF embryos .关键词
体外受精/胚胎质量/自发顶体反应率/密度梯度离心Key words
in vitro fertilization/embryo quality/spontaneous acrosome reaction rate/density gradi-ent centrifugation分类
临床医学引用本文复制引用
刘树沅,韦剑洪,陈伟辉,霍骏业..精子自发顶体反应率与体外受精胚胎质量的相关性研究[J].检验医学与临床,2018,15(1):16-18,3.基金项目
广东省中山市科技计划资助项目(2015B1170). （2015B1170）
