摘要
Abstract
Objective Using Sysmex XN-20 automatic hematology analyzer to detect the percentage of im-mature platelet fraction (IPF％ ) and the absolute number of immature platelet (IPF#) in the peripheral blood , to evaluate the performance of the IPF％ and IPF# and establish a reference interval ,to investigate the clini-cal significance in the thrombocytopenic diseases .Methods The performance of the analyzer for detecting IPF％ and IPF # was evaluated ,including precision ,stability ,carryover and the linear range ,and correlated with Sysmex XE-5000 detection of IPF％ and IPF# .In this research ,240 normal samples were used to form a reference interval and set for negative control group .111 samples of the thrombocytopenic diseases were se-lected as positive group ;Receiver operating characteristic curve between the ITP group and the healthy control group was drawed .Results The within-batch precisions of IPF％ and IPF# for normal-level ,high-level were 2 .52％ ,2 .32％ and 3 .60％ ,1 .93％ ,the between-batch precisions were 1 .8％ and 3 .7％ ;the stability of IPF％ and IPF# for normal-level were 8 .4％ ,8 .8％ within 24 hours ,the high-level were 4 .4％ ,4 .5％ within 24 hours;the carryover of IPF％ was 0 .16％ ,the carryover of IPF # was 0 .27％ ;a linear relation of IPF # was observed from 0 to 94 .8 × 109/L ,which had no statistical difference with Sysmex XE-5000(P>0 .05) .No sta-tistical difference was observed within gender (P>0 .05) ,and the reference interval was determined as IPF％ 0 .9％ -4 .5％ and IPF#(3 .7-9 .7)× 109/L .In idiopathic thrombocytopenic purpura(ITP) group and myelo-dysplastic syndrome(MDS) group ,IPF％ had statistical significance compared with healthy control group (P<0 .05);In aplastic anemia (AA ) ,acute lymphoblastic leukemia (ALL ) and acute nonlymphocytic leukemia (AML) group ,IPF# had statistical significance compared with healthy control group (P<0 .05) .The IPF％ cut-off for a diagnosis of ITP was 11 .6％ ,the sensitivity was 100％ and specificity was 100％ .Conclusion The determination of IPF％ and IPF# by the Sysmex XN-20 have high precision ,good stability ,very low car-ryover and good linear range of the IPF# ,meeting the need of clinical determination ,they have certain clinical application values in the clinical diagnosis ,differential diagnosis and prognosis of the thrombocytopenia dis-ease .关键词
SysmexXN-20全自动血细胞分析仪/未成熟血小板/血小板减少性疾病Key words
Sysmex XN-20 automatic hematology analyser/immature platelet fraction/thrombocyto-penia disease分类
医药卫生
