环境与职业医学2017，Vol.34Issue(12)：1060-1066,7.DOI:10.13213/j.cnki.jeom.2017.17447
补体调节蛋白CD46在聚肌胞苷酸加重三氯乙烯致敏小鼠肝损伤中的表达规律
Expression of complement regulatory protein CD46 in poly I ∶ C exacerbated liver immune injury in trichloroethylene sensitized mice
摘要
Abstract
[Objective] To study the expression and transcription of complement regulatory protein CD46 in immune liver injury of trichlorethylene (TCE) sensitized mice exacerbated by poly I∶ C.[Methods] Thirty-six female BALB/c mice (6-8 weeks old) were randomly divided into blank control group (n=4),solvent control group (n=4),TCE treatment group (n=15),and TCE+poly I ∶ C treatment group (n=13).Sensitization treatment was administered on day 1,4,7,and 10 after adaptive feeding for one week,and challenge on day 17 and 19.The mice in the TCE+poly I ∶ C treatment group were given intraperitoneal injection of 50 μg poly I∶ C at 3 h before last challenge treatment.Mice skin reactions were observed and scored at 24 h after the last challenge.ALT and AST were detected by commercial kit from serum and liver samples at 48 h after challenge.Histological damage of liver was observed by HE staining.Expressions of CD46 and C3 fragment were observed by immunohistochemistry.mRNA expressions of CD46 were detected by RT-PCR.[Results] There was no skin erythema or edema in the blank control group and the solvent control group.The sensitization rate was 33.3％ (5/15) in the TCE treatment group and 38.5％ (5/13) in the TCE+poly I∶ C treatment group,with no statistical differences.The liver/body coefficients in the TCE sensitized group and the TCE+pply I ∶ C sensitized group were significantly higher than that in the solvent control group (P＜ 0.01).No difference in the serum levels of ALT and AST was observed among the blank control group,the solvent control group,and the non-sensitized group.Compared with the solvent control group,the serum levels of ALT and AST in the TCE sensitized group and the TCE+poly I∶C sensitized group were increased (P ＜ 0.01).Compared with the TCE sensitized group,the serum levels of ALT and AST in the TCE+poly I∶C sensitized group were higher (P＜0.01).Pathology examination of liver showed abnormal cell morphology and cell edema in the TCE sensitized group,and a large area of vacuolar degeneration of cells was observed in the TCE+poly I ∶ C sensitized group,leading to liver cell cytoplasm loose and eosin staining lighter.Immunohistochemistry results showed little C3 fragment in TCE sensitized group,but largely deposited in the TCE+poly I ∶ C group,and none in the blank control group,the solvent control group,and the non-sensitized group.The liver immunohistochemical assay results showed high expression of CD46 in liver cell surface in the blank control group,the solvent control group,and the non-sensitized group,reduced expression in the TCE sensitized group,and minimal in the TCE+poly I∶C sensitized group.There was no difference in transcriptions of CD46 among the blank control group,the solvent control group,and the non-sensitized group.However,compared with the solvent control group,the transcription was decreased in the TCE sensitized group and the TCE+poly I ∶ C sensitized group.Compared with the TCE sensitized group,the transcriptions of CD46 in the TCE+poly I ∶ C sensitized group were decreased (P ＜ 0.01).[Conclusion] CD46 may play an important role in poly I∶ C exacerbated immune liver injury in TCE sensitized mice.关键词
三氯乙烯/CD46/聚肌胞苷酸/补体/C3片段Key words
trichloroethylene/CD46/poly I ∶ C/complement/C3 fragment分类
医药卫生引用本文复制引用
李博栋,王贤,杨小东,张澄,杨鹏,臧丹丹,张家祥,朱启星..补体调节蛋白CD46在聚肌胞苷酸加重三氯乙烯致敏小鼠肝损伤中的表达规律[J].环境与职业医学,2017,34(12):1060-1066,7.基金项目
国家自然科学基金(编号:81371730,81673141,81502791) （编号:81371730,81673141,81502791）
