环境与职业医学2017，Vol.34Issue(12)：1093-1097,5.DOI:10.13213/j.cnki.jeom.2017.17403
四种饮用水对小鼠抗氧化功能的影响
Effects of four kinds of drinking water on antioxidant capacity in mice
摘要
Abstract
[Objective] To explore the effects of four kinds of drinking water on antioxidant capacity in mice.[Methods] Eighty ICR mice (half male and half female) were randomly divided into four groups and fed with pure water,comineralized water,tap water,and filtered tap water,respectively,for 90d,with 20 mice in each group.Water quality parameters were detected during the trial including pH value,total dissolved solids (TDS),oxidation-reduction potential (ORP),electrical conductivity (EC),and contents of calcium,magnesium,zinc,copper,manganese,selenium,nitrate radical,and sulfate radical.Antioxidant capability in vitro were also determined,including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging rate,2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging rate,hydroxyl radical scavenging rate,hydroxyl radical scavenging capacity,and anti-superoxide anion activity.After the trial,the total antioxidant capacity (T-AOC),inhibiting abilities of hydroxyl radical and superoxide anion radical,contents of malondialdehyde (MDA) and glutathione (GSH),and activities of superoxide dismutase (SOD),catalase (CAT),peroxidase (POD),glutathione peroxidase (GSH-Px),glutathione S-transferase (GST),glutathione reductase (GR) in liver and kidney samples of the tested mice were also determined.[Results] The inhibiting capabilities of hydroxyl in the pure water,co-mineralized water,tap water,and filtered tap water group were (-11.22 ± 3.78),(-12.37± 1.82),(-16.81 ± 2.88),and (48.33 ± 2.28)U/mL,respectively,and the filtered tap water group showed a higher inhibiting capability of hydroxyl than the others (P＜0.05).In liver tissues,the T-AOC in the tap water group [(4.69 ±0.49) U/mg,in terms of per mg protein,thereafter] was higher than that in the pure water group [(3.06 ± 0.30) U/mg],the activity of GSH-Px in the filtered tap water group [(344.40 ± 14.12) U/mg] was higher than that in the pure water group [(261.72 ± 21.59) U/mg]and the co-mineralized water group [(291.73 ± 10.54)U/mg],and the activity of GST in the tap water group [(1047.56 ± 56.18) U/mg]was higher than that in the pure water group [(776.18 ± 64.97)U/mg] and the co-mineralized water group [(810.40 ± 50.41) U/mg] (P ＜0.05).In kidney tissues,the activity of SOD in the tap water group [(310.24 ± 7.95)U/mg] was higher than that in the pure water group [(274.98 ± 7.80)U/mg],the activity of GSH-Px in the filtered tap water group [(308.54 ± 13.29)U/mg] was higher than that in the pure water group [(252.67 ± 11.30) U/mg],and the activity of GST in the tap water group [(701.63 ± 28.93) U/mg] was higher than that in the co-mineralized water group [(555.63 ± 40.13) U/mg] (P＜ 0.05).[Conclusion] The selected four kinds of drinking water have different effects on antioxidant capacity in mice.Filtered tap water and tap water show potential in improving antioxidant enzyme activities and reducing oxidative stress,while pure water and comineralized water do not.关键词
饮用水/抗氧化/氧化应激Key words
drinking water/antioxidant/oxidative stress分类
医药卫生引用本文复制引用
卢婵,辛凡,温烯楠,孙倩,王敏杰,林郯城,阮国洪..四种饮用水对小鼠抗氧化功能的影响[J].环境与职业医学,2017,34(12):1093-1097,5.基金项目
福建省科技计划项目(编号:2016Y4004) （编号:2016Y4004）
