南京医科大学学报（自然科学版）2017，Vol.37Issue(11)：1515-1518,1523,5.DOI:10.7655/NYDXBNS20171135
兔下颌前牙即刻种植后种植体周围炎动物模型的构建
Establishment of an experimental animal model of peri-implantitis after immediate implantation of mandibular anterior teeth in rabbits
摘要
Abstract
Objective:To establish an experimental animal model of peri-implantitis after immediate implantation of mandibular anterior teeth in rabbits.Methods:A total of 12 healthy male rabbits were anesthetized in the study.A tapered titanium implant was inserted immediately after removal of the left mandibular anterior tooth in each rabbit.Three rabbits were randomly selected and executed after 8 weeks of implantation.Micro-CT was used to evaluate the osseointegration.Then,the rest of animals were randomly divided into three groups.The blank group was not given stimulation,while the control group and the experimental group were injected phosphate buffer solution and E.coli-LPS solution around the implant respectively.After 2 weeks of stimulation,all rabbits were sacrificed to collect the mandible samples with implants and gingival tissues.Micro-CT was used to evaluate bone mass around implants,and hematoxylin and eosin staining was used to observe the histological changes of gingival tissues.Results:After 8 weeks of implantation,satisfactory osseointegration of implants were formed with healthy gums.After 2 weeks of stimulation,the blank and control groups showed pink tough gums with scattered inflammatory cells and good osseointegration of implants without bone resorptions.The experimental group showed red swollen gums with massive inflammatory cells and apparent bone resorptions on the top of implants with reduced bone mineral density and percent bone volume.Conclusion:An experimental animal model of peri-implantitis in rabbits could be established rapidly by local injections of E.coli-LPS solution around the implant.关键词
兔/即刻种植/种植体周围炎/原位动物模型Key words
rabbit/immediate implantation/peri-implantitis/animal model分类
医药卫生引用本文复制引用
虞颖娟,明盼盼,邱憬..兔下颌前牙即刻种植后种植体周围炎动物模型的构建[J].南京医科大学学报（自然科学版）,2017,37(11):1515-1518,1523,5.基金项目
国家自然科学基金(81472928) （81472928）
江苏省卫生计生委面上科研课题(H201641) （H201641）
江苏省青年医学人才项目(QNRC-2016850) （QNRC-2016850）
江苏省“六大人才高峰”资助项目(2014-WSW-035) （2014-WSW-035）
江苏省高校大学生创新计划项目(201310312020Z) （201310312020Z）
江苏高校优势学科建设工程资助项目(2014-37) （2014-37）
