中国海洋大学学报（自然科学版）2017，Vol.47Issue(11)：31-39,9.DOI:10.16441/j.cnki.hdxb.20160208
外源寡核苷酸对大肠杆菌和双歧杆菌生长的影响
The Influence of Exogenous Oligonucleotides on the Growth of Escherichia coli and Bifidobacterium adolescentis
摘要
Abstract
The safety and function of dietary nucleic acids on human body have received significant attentions in recent years.Adding exogenous nucleotides was widely recommended in infant formula milk powder and infant food for their beneficial functions.In the past,the researches mainly focused on the effect of mononucleotides on intestinal microorganisms.The nucleic acids are taken in mostly through food,and oligonucleotides are the important form of intake nucleic acids.However,there was less study on the influence of oligonucleotides on the intestinal microorganisms.In this study,we employed two kinds of oligonucleotides to investigate their effects on the growth of two typical intestinal bacteria Escherichia coli and Bifidobacteriumadolescentis.Firstly,E.coli and B.adolescentis were cultured separately in the medium lacking nucleic acids,and then two kinds of oligonucleotides (39 nt and 61 nt in length) were added into the medium.The primary results showed that the two oligonucleotides promoted the growth of E.coli and B.adolescentis,respectively.Considering the complex intestinal bacterial community,E.coli and B.adolescentis were then culture together,and real time quantitative PCR was used to quantifying the 16S rRNA gene (rDNA) of the two bacteria.Compared with the control,the added oligonucleotides promoted the B.adolescentis growth.However,it inhibited the growth of E.coli obviously.To study the form of the oligonucleotides appropriate for bacteria,the collected bacteria were broken with ultrasonic wave with the released cytoplasm mixed with the oligonucleotides in order to simulate the existence of oligonucleotides in bacteria.The results of electrophoresis showed that the oligonucleotides remained undestroyed in the medium;however,they were degradable in bacterial cytoplasm.The results also showed that the degradation rate of 39 nt oligonucleotide was faster than that of the 61 nt oligonucleotide in both bacteria.The results indicated that the intestinal microorganisms preferred to degrade oligonucleotides instead of the integrated ones.In conclusion,intestinal microorganisms were able to utilize the exogenous nucleic acids for their growth.Meanwhile,exogenous nucleic acids play a positive role in maintaining the balance of gut microbes.关键词
寡核苷酸/肠道微生物/双歧杆菌/大肠杆菌Key words
oligonucleotides/intestinal microorganism/Bifidobacterium adolescentis/Escherichia coli分类
轻工纺织引用本文复制引用
高晓梦,苏健,王晓倩,李玉芝,孔青,董平,梁兴国..外源寡核苷酸对大肠杆菌和双歧杆菌生长的影响[J].中国海洋大学学报（自然科学版）,2017,47(11):31-39,9.基金项目
国家自然科学基金项目(31201327,31301420) （31201327,31301420）
国家自然科学基金委员会-山东省人民政府联合资助海洋科学研究中心项目(U1406402)资助 Supported by National Natural Science Foundation of China(31201327,31301420) （U1406402）
National Natural Science Foundation of China(NS-FC)-Shandong Joint Fund for Marine Science Research Centers(U1406402) （NS-FC）
