热带作物学报2017，Vol.38Issue(9)：1601-1606,6.DOI:10.3969/j.issn.1000-2561.2017.09.004
EMS诱变苦荞突变体与其亲本的比较分析
Comparative Analysis of Fagopyrum tataricum Mutants and Its Parents in EMS Mutagenesis
摘要
Abstract
The protein,flavonoid,soluble sugar,total chlorophyll contents and the activity of peroxidase (POD),catalase (CAT),superoxide dismutase (SOD) of the mutants of Fagopyrum tataricum including Dwarf mutants (M1),small-grain mutants (M2),early-maturity mutant (M3),late-maturity mutants (M4),big-leaf mutants (M5) of were studied by means of Kjeldahl,AlCl3,anthrone colorimetry,spectrophotometry,guaiacol,KMn04 titration,nitro blue tetrazolium (NBT) and photo-reduction methods.The results showed that the flavonoid contents and the CAT activity of M1 were significantly higher than that of CK,POD activity significantly was lower than that of CK.However,the differences of protein,soluble sugar,total chlorophyll and SOD activity were not significant with that of CK.The flavonoid,soluble sugar,total chlorophyll contents and the CAT activity of M2 were significantly higher than that of CK,and the protein contents and the POD activity of M2 were not significantly with that of CK.While the activity of M2 was significantly lower thanthat of CK.The CAT and SOD activity of M3 were significantly lower than that of CK,while the protein,flavonoid,soluble sugar,total chlorophyll contents and the POD activity were no significant difference from that of CK.The protein contents of M4 were significantly higher than that of CK,and the CAT activity was no significant different.The protein contents of M5 were no significant difference from that of CK.But its CAT activity were significantly higher than that of CK.Whereas the contents of flavonoid,soluble sugar,total chlorophyll,POD and SOD were higher than that of CK.关键词
荞麦突变体/蛋白质/黄酮/可溶性糖/POD/SOD/CAT/叶绿素Key words
Buckwheat mutants/protein/flavonoid/soluble sugar/POD/CAT/SOD/chlorophyll分类
农业科技引用本文复制引用
张以忠,邓琳琼..EMS诱变苦荞突变体与其亲本的比较分析[J].热带作物学报,2017,38(9):1601-1606,6.基金项目
中央高校基本科研业务费专项资金资助(No.XDJK2012C111) （No.XDJK2012C111）
贵州省科技厅、毕节市科技局及毕节学院科技联合基金(黔科合J字LKB [2013] 21号) （黔科合J字LKB [2013] 21号）
贵州省科技厅、毕节市科技局及贵州工程应用技术学院科技联合基金(黔科合LH字LKB [2014] 7540号) （黔科合LH字LKB [2014] 7540号）
贵州省教育厅青年科技人才成长项目(黔教合KY字[2016] 286). （黔教合KY字[2016] 286）
