热带作物学报2017，Vol.38Issue(9)：1671-1676,6.DOI:10.3969/j.issn.1000-2561.2017.09.015
羽裂报春苣苔的组织培养研究
Tissue Culture of Primulina pinnatifida
摘要
Abstract
With the leaves of Primulina pinnatifida as the explants,the tissue culture technique system of P.pinnatifida was studied based on the effects of sterilization methods,plant growth regulator types and concentrations.The results showed that the suitable method for the sterilization of the explants was the leaves were sterilized in 75％ alcohol for 20 seconds,then soaked in 0.1％ HgCl2 for 6 minutes,and soaked in 0.1％ HgCl2 for another 6 minutes.The survival rate of the explants was 55.40％.The suitable culture medium for adventitious bud induction was MS+6-BA 1.0-2.0 mg/L+NAA 0.01-0.1 mg/L,the most suitable culture medium for adventitious bud multiplication was MS+6-BA 2.0 mg/L+NAA 0.05 mg/L+activated carbon 0.1 g/L,and the multiplication coefficient was 8.61.The optimum culture medium for rooting culture was MS+IBA 0.5 mg/L+activated carbon 0.1 g/L,and the rooting rate was 100％.For the cultivation,the suitable ratio of transplanting medium of the in vitro plantlets was peat∶perlite∶ sand 4 ∶ 1 ∶ 1 (V ∶ V ∶ V),and the survival rate of transplanting of the in vitro plantlets reached 99.60％.关键词
羽裂报春苣苔/组织培养/二次灭菌/活性炭Key words
Primulina pinnatifida/tissue culture/secondary sterilization/activated carbon分类
农业科技引用本文复制引用
闫海霞,关世凯,黄昌艳,邓杰玲,何荆洲,张自斌,卜朝阳..羽裂报春苣苔的组织培养研究[J].热带作物学报,2017,38(9):1671-1676,6.基金项目
广西科学研究与技术开发计划项目(桂科攻1598006-5-8,桂科能1598022-1-5) （桂科攻1598006-5-8,桂科能1598022-1-5）
南宁市科学研究与技术开发计划项目(No.NC20152008-3) （No.NC20152008-3）
南宁市西乡塘区科学研究与技术开发计划项目(No.2015302) （No.2015302）
广西农科院项目(农成转2015009,2015JM03,2015YT89). （农成转2015009,2015JM03,2015YT89）
