四川大学学报（自然科学版）2018，Vol.55Issue(1)：214-220,7.DOI:10.3969/j.issn.0490-6756.2018.01.035
香菇UDP-葡萄糖焦磷酸化酶转录水平及酶活性对不同碳氮源的响应特征
Transcriptional expression profiles and enzyme activity of UGP from Letinous edodes under different carbon and nitrogen sources
摘要
Abstract
Different carbon and nitrogen sources were used to culture the Lentinula edodes Xin808 in this study .The contents of intracellular polysaccharide were determined by phenol-sulfuric acid .The expres-sion level of UDP-glucose pyrophosphorylase ,UGP gene were analyzed by real-time quantitative PCR , and enzyme activity of UGP were also determined .The results showed that ,comparing to the control , besides NH4NO3 treatment group ,the rest of carbon and nitrogen sources could increase the biomass of Letinous edodes ,among which wheat bran exhibited as the best (0 .63g) .The small molecule (sucrose , maltose and mannose) as carbon sources and organic compounds(wheat bran and yellow bean sprouts ) as nitrogen sources ,UGP gene relative expression level obviously were up-regulated ,increased UGP activ-ity ,promoted the biosynthesis of mycelium intracellular polysaccharide .With sucrose as carbon source and wheat bran as nitrogen source ,the relative expression level of UGP gene ,the activity of UGP and mycelium polysaccharide content were the highest .The expression level of UGP gene ,the activity of UGPase and mycelium intracellular polysaccharide of Letinous edodes showed high positive correlation between the three .The small molecule carbon sources (sucrose ,maltose and mannose) and organic ni-trogen sources (wheat bran and yellow bean sprouts) were helpful to the biosynthesis of mycelium poly-saccharide .Sugar and wheat bran could be used as advantage carbon source and nitrogen source under Letinous edodes liquid fermentation .关键词
香菇多糖/UDP-葡萄糖焦磷酸化酶/酶活性/基因相对表达量Key words
Lentinan/UDP-glucose pyrophosphorylase/Engyme activity/Relative expression quantity分类
生物科学引用本文复制引用
陈小敏,吴海冰,向泉桔,辜运富,张小平..香菇UDP-葡萄糖焦磷酸化酶转录水平及酶活性对不同碳氮源的响应特征[J].四川大学学报（自然科学版）,2018,55(1):214-220,7.基金项目
四川省"十三五"育种攻关项目(2016NYZ0040) （2016NYZ0040）
