四川环境2017，Vol.36Issue(6)：36-44,9.
土地利用方式改变以及长期施肥处理对酸性土壤微生物群落结构的影响
The Impact of Land Use Change and Long-term Fertilization on the Microbial Communities in Acidic Soils
摘要
Abstract
Microbial communities inhabiting in soils are one of the crucial players in maintaining health and balance of the soil ecosystem.The impact of different land use and fertilization on soil microbial community structure is significant.This study aimed to evaluate the microbial community structure and diversity in upland and paddy soil which with fertilization (US/PS-NPK) or without fertilization (US/PS-CK).These soils have been managed for 20 years.Using the 16S rRNA-based pyrosequencing,we compared and analyzed the microbial community structure and diversity,and did correlation analysis combining with environmental factors.The results showed that the order of diversity of microbial communities was as follows:US-NPK ＜ US-CK ＜ PS-CK ＜ PS-NPK,which indicating the shift of upland soil to paddy soil increased the microbial diversity,and the influence of land use change on microbial diversity is significantly higher than that of long term fertilization.The dominant flora of the four kinds of soils is consistent with normal soil,which are Chloroflexi (22.0％ ～30.3％),Proteobactria (18.2％ ～32.7％),Acidobnctria (19.7％ ～ 24.3％),and Actinobnctria (6.0％ ～ 13.6％).However,the metabolic function of the microbial communities in the four kinds of soil showed difference.The study further revealed the responding mechanism of soil microbes under ecological environment change,,and provides the scientific basis for the improvement of fertilization and farming system关键词
土地利用方式/施肥/微生物群落结构/酸性土壤/高通量测序Key words
Land use type/fertilization/microbial communities/acidic soil/pyrosequencing分类
农业科技引用本文复制引用
张静,李会琳,王岚,杨宏,何燕,路璐..土地利用方式改变以及长期施肥处理对酸性土壤微生物群落结构的影响[J].四川环境,2017,36(6):36-44,9.基金项目
国家自然科学基金项目(41606142) （41606142）
西华师范大学科研启动基金(412554). （412554）
