山东农业科学2017，Vol.49Issue(10)：15-19,5.DOI:10.14083/j.issn.1001-4942.2017.10.003
利用ARMS-Tm-shift-qPCR技术检测水稻低直链淀粉含量基因Wx-mq
Detection of Wx-mq Gene for Low-Amylose Content by ARMS-Tm-shift-qPCR Technology
摘要
Abstract
Wx-mq gene is a low amylose trait controlled gene and closely relates with the eating quality of rice.According to the rice low amylose starch content control gene Wx-mq No.497 position existing G ＞A single nucleotide variants,two real-time PCR genotyping methods,ARMS and Tm-shift based on SYBR Green Ⅰ,was combined to establish the fluorescence real-time quantitative PCR system,which could accurately distinguish the GG homozygote,AA homozygote and GA heterozygosis of Wx-mq gene No.497 position.The experiment results showed that the ARMS-Tm-shift real-time PCR genotyping method based on SYBR Green Ⅰ did not need probe synthesis with simple designs.It was a rapid,simple and specific genotyping method.It could be used in high-throughput genotyping of Wx-mq gene in molecular marker assisted breeding.关键词
水稻/Wx-mq基因/低直链淀粉含量/ARMS-Tm-shift-qPCRKey words
Rice/Wx-mq gene/Low-amylose content/ARMS-Tm-shift-qPCR分类
农业科技引用本文复制引用
陈峰,徐建第,姜明松,张全芳,朱文银,李广贤,周学标,杨连群..利用ARMS-Tm-shift-qPCR技术检测水稻低直链淀粉含量基因Wx-mq[J].山东农业科学,2017,49(10):15-19,5.基金项目
国家重点研发计划项目(2017YFD0100505) （2017YFD0100505）
国家水稻产业体系济宁综合试验站(CARS-01-60) （CARS-01-60）
国家科技支撑计划子课题(2015BAD01B02-1-3) （2015BAD01B02-1-3）
山东省水稻产业体系项目(SDAIT-17-01) （SDAIT-17-01）
山东省重点研发计划项目(2017GNC11111) （2017GNC11111）
山东省农业科学院科技创新重点项目(2014CXZ10-5) （2014CXZ10-5）
山东省农业科学院科技创新工程项目(CXGC2016A11) （CXGC2016A11）
山东省农业科学院重大成果培育项目(2016CGPY09) （2016CGPY09）
