猪圆环病毒2型LAMP检测方法的建立和初步应用OACSCDCSTPCD
Establishment and preliminary application of LAMP for detection of porcine circovirus type 2
根据GenBank上已报道的猪圆环病毒2型(PCV2)基因组序列设计1组LAMP引物(F3/B3、FIP/BIP),通过对环介导等温扩增技术(LAMP)反应条件的优化,建立了猪圆环病毒2型LAMP检测方法,并对阳性结果进行了酶切验证.结果表明:该方法具有较高的检测灵敏度,最低可以检测到反应体系内1 pg的PCV2DNA;通过反应结束后的肉眼可视化观察,可方便地进行结果判定.本研究建立的PCV2 LAMP检测方法能够满足基层临床PCV2病原的快速检测要求.
A group of LAMP primers(F3/B3,FIP/BIP)were designed according to the genome sequences of porcine circovirus type 2 (PCV2) reported on GenBank.The LAMP method for detecting porcine circovirus type 2 was established by optimizing the reaction conditions of loop mediated isothermal amplification(LAMP),and the positive results were verified by enzyme digestion.The results showed that the method had high detection sensitivity,and the PCV2 DNA of 1 pg in the…查看全部>>
高骏;易建中;李红;刘成倩;姚惠娟;孙凤萍
上海市农业科学院畜牧兽医研究所,上海201106上海市农业科学院畜牧兽医研究所,上海201106上海市农业科学院畜牧兽医研究所,上海201106上海市农业科学院畜牧兽医研究所,上海201106上海市农业科学院畜牧兽医研究所,上海201106上海市农业科学院畜牧兽医研究所,上海201106
农业科技
猪圆环病毒2型LAMP可视化
Porcine circovirus type 2LAMPVisualization
《上海农业学报》 2017 (5)
31-34,4
上海市科委项目:猪繁殖与综合征病毒和猪圆环病毒2型LAMP基因快速检测方法的建立(14140900703)
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